Streptomyces aquilus sp. nov., a novel actinomycete isolated from a Chinese medicinal plant. Issue 3 (22nd January 2020)

• Record Type: Journal Article
• Authors: Li, Kaiqin; Guo, Yihui; Wang, Junzhen; Wang, Zhiyong; Zhao, Jiarong; Gao, Jian
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 70:Issue 3(2020)
• Page Start: 1912
• Page End: 1917
• Publication Date: 2020-01-22
• Subjects: Streptomyces aquilus sp. nov. -- Xanthium sibiricum -- Polyphasic taxonomy; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.003995
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : The taxonomic position of a novel actinomycete isolate, designated strain GGCR-6 T, isolated from the healthy leaves of Xanthium sibiricum collected from the botanic garden of Hunan University of Science and Technology in Hunan province, PR China, was determined by a polyphasic approach. GGCR-6 T grew well on ISP series media and formed well-developed, branched substrate hyphae and aerial mycelium that differentiated into straight spore chains consisting of cylindrical spores with smooth surfaces. The diagnostic diamino acid was ll -diaminopimelic acid. The major menaquinones were MK-9(H8 ), MK-9(H2 ), MK-9 and MK-9(H6 ). The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphotidylinositol and phosphatidylinositol mannosides. The predominant fatty acids were C16 : 1 ω9 c, iso-C16 : 0 and C16 : 0 . The phenotypic characteristics of GGCR-6 T indicated that it represented a member of the genus Streptomyces . Phylogenetic analysis based on the 16S rRNA gene sequence indicated that GGCR-6 T was most closely related to Streptomyces cyaneus NRRL B2296 T and Streptomyces griseoruber NRRL B1818 T . However, the digital DNA–DNA hybridization, the average nucleotide identity and the multi locus sequence analysis evolutionary distance clearly separate GGCR-6 T from the phylogenetically closely related species. Furthermore, the novel isolate was distinctly differentiated from S. cyaneus NRRL B2296 T and S. griseoruber NRRL B1818 T by morphological, physiological and biochemical characteristics. Based on these data, strain GGCR-6 T should be designated as a representative of a novel species of the genus Streptomyces, for which the name Streptomyces aquilus sp. nov. is proposed. The type strain is strain GGCR-6 T (=CICC 11055 T =JCM 33584 T ).