Chronic stress induces cell type-selective transcriptomic and electrophysiological changes in the bed nucleus of the stria terminalis. (15th May 2019)

• Record Type: Journal Article
• Authors: Daniel, Sarah E.; Menigoz, Aurélie; Guo, Jidong; Ryan, Steven J.; Seth, Shivani; Rainnie, Donald G.
• Is Part Of: Neuropharmacology. Volume 150(2019)
• Page Start: 80
• Page End: 90
• Publication Date: 2019-05-15
• Subjects: Bed nucleus of the stria terminalis -- Stress -- Single-cell RT-PCR -- AMPA receptors -- Corticotropin releasing factor; adBNST anterodorsal bed nucleus of the stria terminalis -- AMPAR:NMDAR AMPA-to-NMDA ratio -- ASR acoustic startle response -- BNST bed nucleus of the stria terminalis -- BNSTALG anterolateral group of the BNST -- CP-AMPAR calcium-permeable AMPAR -- CRF corticotropin releasing factor -- CSS chronic shock stress -- EPSC excitatory postsynaptic current -- EZM elevated zero maze -- LTD long-term depression -- LTP long-term potentiation -- 1-naphthylacetyl spermine trihydrochloride NASPM -- NS non-stressed -- ovBNST oval nucleus of the BNST -- PVN paraventricular nucleus of the hypothalamus -- scRT-PCR single-cell reverse transcriptase PCR -- SEFL stress-enhanced fear learning -- STEP striatal-enriched protein tyrosine phosphatase; NASPM (PubChem CID: 129695); Neuropsychopharmacology -- Periodicals; Autonomic Agents -- Periodicals; Neuropsychopharmacologie -- Périodiques; Neuropsychopharmacology; Periodicals; Electronic journals; 615.78
• DOI: 10.1016/j.neuropharm.2019.03.013
• Languages: English
• ISSNs: 0028-3908

Abstract

Abstract: Distinct regions and cell types in the anterolateral group of the bed nucleus of the stria terminalis (BNSTALG ) act to modulate anxiety in opposing ways. A history of chronic stress increases anxiety-like behavior with lasting electrophysiological effects on the BNSTALG . However, the opposing circuits within the BNSTALG suggest that stress may have differential effects on the individual cell types that comprise these circuits to shift the balance to favor anxiogenesis. Yet, the effects of stress are generally examined by treating all neurons within a particular region of the BNST as a homogenoeus population. We used patch-clamp electrophysiology and single-cell quantitative reverse transcriptase PCR (scRT-PCR) to determine how chronic shock stress (CSS) affects electrophysiological and neurochemical properties of Type I, Type II, and Type III neurons in the BNSTALG . We report that CSS resulted in changes in the input resistance, time constant, action potential waveform, and firing rate of Type III but not Type I or II neurons. Additionally, only the Type III neurons exhibited an increase in Crf mRNA and a decrease in striatal-enriched protein tyrosine phosphatase ( Ptpn5 ) mRNA after CSS. In contrast, only non-Type III cells showed a reduction in calcium-permeable AMPA receptor (CP-AMPAR) current and changes in mRNA expression of genes encoding AMPA receptor subunits after CSS. Importantly, none of the effects of CSS observed were seen in all cell types. Our results suggest that Type III neurons play a unique role in the BNSTALG circuit and represent a population of CRF neurons particularly sensitive to chronic stress. Highlights: Chronic shock stress (CSS) induces cell-type selective changes in neurons of the anterolateral BNST. CSS selectively alters electrophysiological properties, as well as CRF and STEP mRNA expression in Type III neurons. CSS induces changes in the CP-AMPAR current and expression of encoding genes only in non-Type III neurons.