Improved method for immunoperoxidase detection of membrane antigens in frozen sections. Issue 8 (August 1989)

• Record Type: Journal Article
• Authors: Green, M A; Sviland, L; Malcolm, A J; Pearson, A D
• Is Part Of: Journal of clinical pathology. Volume 42:Issue 8(1989)
• Page Start: 875
• Page End: 880
• Publication Date: 1989-08
• Subjects: Pathology -- Periodicals; Pathology, Molecular -- Periodicals; 616.0705
• DOI: 10.1136/jcp.42.8.875
• Languages: English
• ISSNs: 0021-9746

Abstract

Abstract : The visualisation of membrane antigens in frozen sections using metal-enhanced 3, 3'diaminobenzidine (DAB) was investigated. Particular attention was paid to the degree of reaction enhancement, the disruptive effect on morphological detail, and the ease of the techniques. The best result was obtained using nickel-modified DAB at pH 6.0 to develop the peroxidase enzyme, with further enhancement in cobalt chloride at neutral pH. Silver methenamine enhancement is also possible but can give rise to non-specific or background staining.