Temporal lobe epilepsy: Evaluation of central and systemic immune-inflammatory features associated with drug resistance. (October 2021)

• Record Type: Journal Article
• Authors: Toledo, Andrea; Orozco-Suárez, Sandra; Rosetti, Marcos; Maldonado, Lorenzo; Bautista, Sara I.; Flores, Ximena; Arellano, Alfonso; Moreno, Sergio; Alonso, Mario; Martínez-Juárez, Iris E.; Fragoso, Gladis; Sciutto, Edda; Fleury, Agnès
• Is Part Of: Seizure. Volume 91(2021)
• Page Start: 447
• Page End: 455
• Publication Date: 2021-10
• Subjects: Temporal lobe epilepsy -- Drug-resistant epilepsy -- Neuroinflammation -- Peripheral inflammation; PWE Patients with epilepsy -- DR Drug resistant epilepsy -- TLE Temporal lobe epilepsy -- DR-TLE Drug resistant temporal lobe epilepsy -- non-DR-TLE Non drug resistant temporal lobe epilepsy -- HC Healthy controls -- HS Hippocampal sclerosis; Epilepsy -- Periodicals; Epilepsy -- Periodicals; Seizures -- Periodicals; Épilepsie -- Périodiques; Electronic journals; Electronic journals; 616.853
• DOI: 10.1016/j.seizure.2021.07.028
• Languages: English
• ISSNs: 1059-1311

Abstract

Highlights: DR-TLE patients exhibited a peripheral decrease in regulatory T and B cells. DR-TLE patients presented a peripheral increase in IL-6 and IL-5. Neuroinflammatory features were observed in some of the DR-TLE patients. Neuroinflammation was positively correlated with peripheral regulatory response. A better understanding of link between compartments could help to improve treatment Abstract: Neuroinflammation is probably one of the factors involved in drug resistance in people with epilepsy. Finding peripheral markers reflecting the intensity of neuroinflammation could be of great help to decide for which patients anti-inflammatory treatment might be an option. In this context, peripheral cytokines levels and lymphocyte phenotypes were assessed by ELISA and flow cytometry in 3 groups of subjects: drug resistant patients with temporal lobe epilepsy (DR-TLE), non DR-TLE patients and healthy controls. The same parameters were assessed in brain tissue in the DR-TLE group. Differences in the peripheral immune-inflammatory status between the 3 groups of subjects, and correlations between the central and peripheral immune-inflammatory status in DR-TLE patients were evaluated. Forty-one patients with DR-TLE, ten with non-DR-TLE and twenty controls were included. In the periphery, decrease in regulatory cells were observed in DR-TLE patients compared to controls. In addition, significant increase of IL-6 and IL-5 was observed in patients with epilepsy (particularly DR-TLE patients). Two groups of DR-TLE patients with significant differences in several central inflammatory parameters were identified in a cluster analysis. The inflammatory cluster was associated with a peripheral increase of CD4+CD38+ cells and different significant correlations between central and systemic inflammatory parameters were observed. Although their interpretation is not immediate, they demonstrate a clear dialogue between central and peripheral inflammatory reactions. In conclusion, our results add new elements to better understand the interactions between the central and peripheral compartments in patients with DR-TLE, and to help better define treatment options in this group of patients. Graphical abstract: Image, graphical abstract