54 Anti-glioma activity of Rana temporaria chensinensis egg protein hydrolysate. (19th December 2016)

• Record Type: Journal Article
• Authors: Liu, Da; Jin, Ye; Li, Yiping
• Is Part Of: Journal of investigative medicine. Volume 64(2016)Supplement 8
• Page Start: A19
• Page End: A19
• Publication Date: 2016-12-19
• Subjects: Clinical medicine -- Periodicals; Medicine -- Research -- Periodicals; Medicine; Research -- United States; Clinical medicine; Medicine -- Research; Periodicals; 616.075
• DOI: 10.1136/jim-2016-000328.54
• Languages: English
• ISSNs: 1081-5589

Abstract

Abstract : Objectives: Rana temporaria chensinensis only live in the Changbai mountain area in the northeast of China. The eggs of R. temporaria chensinensis contain many special ingredients that modulate incretion. This study aimed to investigate the effects of R. temporaria chensinensis egg protein hydrolysate on human glioma C6 cell proliferation and apoptosis. Methods: We extracted R. temporaria chensinensis egg protein (500 mg/mL) and investigated its effects on human glioma C6 cell cultures, and subjected it to an MTT assay, colony forming assay, Western blot assay and flow cytometry analysis of apoptosis. We further investigated the effects of R. temporaria chensinensis egg protein hydrolysate (1.5 g/kg) on glioma development and progression in vivo using a mouse model of glioma. Results: R. temporaria chensinensis egg protein hydrolysate inhibited the proliferation of glioma cells; these effects were mediated by the phosphoinositide 3-kinase (PI3K)/AKT signalling pathway. Conclusions: This study suggested that R. temporaria chensinensis egg protein hydrolysate promotes apoptosis of glioma cells both in vitro and in vivo. Acknowledgments: This research was financially supported by Jilin Province Science and Technology Development Program (Grant Nos. 20150309008YY and 20150309009YY).