Activation of RAW264.7 cells by PCp-I, a polysaccharide from Psoralea corylifolia L, through NF-κB/MAPK signalling pathway. (April 2021)

• Record Type: Journal Article
• Authors: Wang, Honglin; Xu, Xiaoqing; Yin, Zhenhua; Wang, Mengke; Wang, Baoguang; Ma, Changyang; Wang, Jinmei; Kang, Wenyi
• Is Part Of: International journal of immunopathology and pharmacology. Volume 35(2021)
• Publication Date: 2021-04
• Subjects: Psoralea corylifolia L -- polysaccharide -- immunomodulation -- RAW264.7 cells; Immunopathology -- Periodicals; Pharmacology -- Periodicals; Inflammation -- Periodicals; 615.1
• DOI: 10.1177/20587384211010058
• Languages: English
• ISSNs: 0394-6320

Abstract

PCp-I is a polysaccharide isolated and identified from the Psoralea corylifolia L. by our research group. In this study, the immunomodulatory effects of PCp-I on RAW264.7 cells was evaluated. PCp-I could enhance the level of NO along with up-regulation of iNOS mRNA in RAW264.7 cells. The PCp-I could significantly up-regulate the mRNA expression of TNF- α and IL-6 in RAW264.7 cells, and then the expression of TNF- α, IL-6, ROS and the phagocytic activity were increased. Additionally, PCp-I could significantly up-regulate the phosphorylation level of p65, p38, ERK and JNK proteins, which proved that PCp-I could activate the macrophages by MAPKs and NF- κ B signalling pathway and the TLR4 may be one of the receptors of PCp-I regulate the RAW264.7 cells.