OIP5-AS1 specifies p53-driven POX transcription regulated by TRPC6 in glioma. (28th January 2021)

• Record Type: Journal Article
• Authors: Shao, Wei; Hao, Zhen-Yu; Chen, Yi-Fei; Du, Jun; He, Qian; Ren, Liang-Liang; Gao, Yan; Song, Nan; Song, Yan; He, Hua; Wang, Yi-Zheng
• Editors: Lu, Hua
• Is Part Of: Journal of molecular cell biology. Volume 13:Number 6(2021)
• Page Start: 409
• Page End: 421
• Publication Date: 2021-01-28
• Subjects: DNA‒RNA hybrid -- glioma -- lncRNA -- p53 -- transcription; Molecular biology -- Periodicals; 571.605
• DOI: 10.1093/jmcb/mjab001
• Languages: English
• ISSNs: 1674-2788

Abstract

Abstract: Transcription factors (TFs) control an array of expressed genes. However, the specifics of how a gene is expressed in time and space as controlled by a TF remain largely unknown. Here, in TRPC6-regulated proline oxidase 1 (POX) transcription in human glioma, we report that OIP5-AS1, a long noncoding RNA, determines the specificity of p53-driven POX expression. The OIP5-AS1 /p53 complex via its 24 nucleotides binds to the POX promoter and is necessary for POX expression but not for p21 transcription. An O-site in the POX promoter to which OIP5-AS1 binds was identified that is required for OIP5-AS1 /p53 binding and POX transcription. Blocking OIP5-AS1 binding to the O-site inhibits POX transcription and promotes glioma development. Thus, the OIP5-AS1 /O-site module decides p53-controlled POX expression as regulated by TRPC6 and affects glioma development.