Pontivivens ytuae sp. nov., isolated from deep sea sediment of the Mariana Trench. Issue 9 (14th September 2021)

• Record Type: Journal Article
• Authors: Wang, Zhen; Du, Rong-bin; Sun, Qing-lei; Sun, Yuan-yuan; Zhang, Jian; Tang, Yong-zheng
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 71:Issue 9(2021)
• Publication Date: 2021-09-14
• Subjects: Mariana Trench -- Pontivivens -- sediment -- Rhodobacteraceae; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.004967
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : A Gram-stain-negative, light pink-coloured, rod-shaped, flagellated and facultative anaerobic bacterial strain, designated MT2928 T, was isolated from deep-sea sediment collected from the Mariana Trench. Growth of strain MT2928 T occurred optimally at 28 °C, pH 8.0–9.0 and in the presence of 1.0–2.0 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain MT2928 T belongs to the genus Pontivivens and has the highest sequence similarity to Pontivivens insulae GYSW-23 T (96.6 %). Genomic analysis indicated that strain MT2928 T contains a circular chromosome of 4 199 362 bp with G+C content of 67.2 mol%. The strain did not produce bacteriochlorophyll a, but produced carotenoid. The predominant respiratory quinone of MT2928 T was ubiquinone-10. The polar lipids of MT2928 T contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, two unidentified lipids and two unidentified phospholipids. The major fatty acids of strain MT2928 T contained summed feature 8 (C18 : 1 ω 7 c or/and C18 : 1 ω 6 c ), C18 : 0 and summed feature 2 (iso-C16 : 1 I and/or C14 : 0 3-OH). On the basis of phylogenetic, physiological, biochemical and other phenotypic properties, strain MT2928 T represents a novel species of the genus Pontivivens, and the name Pontivivens ytuae sp. nov. is proposed with the type species MT2928 T (=MCCC 1K05575 T =JCM 34320 T ).