Ameliorating effects of white mulberry on iron-overload-induced oxidative stress and liver fibrosis in Swiss albino mice. (October 2021)

• Record Type: Journal Article
• Authors: Shendge, Anil Khushalrao; Panja, Sourav; Basu, Tapasree; Ghate, Nikhil Baban; Mandal, Nripendranath
• Is Part Of: Food and chemical toxicology. Volume 156(2021)
• Publication Date: 2021-10
• Subjects: Iron -- Morus alba -- Free radicals -- Fruit -- Biomarkers -- GC-MS; Toxicology -- Periodicals; Food poisoning -- Periodicals; Food Poisoning -- Periodicals; Toxicology -- Periodicals; Toxicologie -- Périodiques; Intoxications alimentaires -- Périodiques; Food poisoning; Toxicology; Periodicals; Electronic journals; 615.9
• DOI: 10.1016/j.fct.2021.112520
• Languages: English
• ISSNs: 0278-6915

Abstract

Abstract: Excess iron causes oxidative damage of biomolecules, leading to tissue injury primarily liver failure. In this study, we explored the remediating effects of Morus alba L. (MAME) on iron-overload-induced oxidative stress and liver injury in mice. The In vitro study revealed the antioxidant and free radical scavenging properties of MAME. Intraperitoneal injection of iron-dextran was administered in Swiss albino mice to induce iron-overload condition and the mice were further treated with MAME. MAME treatment significantly decreased liver iron, serum ferritin level, oxidative stress, and restored serum parameters and liver antioxidants. Moreover, biochemical and histopathological analyses confirmed the alleviated liver damage and fibrosis upon MAME treatment. The protective effect of MAME against iron-overload-induced apoptosis was confirmed by upregulation of protein levels of Bax, Caspase-3, and PARP. The treatment also affected the expression of MAPKs (ERK, JNK, and p38). GC-MS analysis revealed the presence of various bioactive phytochemicals in MAME that may be responsible for ameliorating effects of excess iron. Thus MAME can be envisaged as an effective iron chelator in the treatment of iron-overload-induced liver injury and fibrosis. Graphical abstract: Image 1 Highlights: White berry, Morus alba (MAME) showed in vitro free radical scavenging and promising iron chelation properties Intraperitoneal injection of iron is associated with oxidative stress, liver fibrosis, and activation of apoptotic signaling MAME ameliorates iron-induced oxidative stress and liver fibrosis in mice model MAME treatment restores serum markers, antioxidant enzymes and rescued the hepatocytes from apoptosis GC-MS analysis showed the presence of active phytochemicals