Microbial cellulases – An update towards its surface chemistry, genetic engineering and recovery for its biotechnological potential. (November 2021)

• Record Type: Journal Article
• Authors: Paul, Manish; Mohapatra, Sonali; Kumar Das Mohapatra, Pradeep; Thatoi, Hrudayanath
• Is Part Of: Bioresource technology. Volume 340(2021)
• Publication Date: 2021-11
• Subjects: Microbial cellulose -- Surface chemistry -- In silico study -- Cellulase engineering -- Cellulase recovery; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2021.125710
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: Surface charge of biomass and cellulases impacts the absorption of enzymes. Secondary structure stability in immobilized cellulases affects catalytic activity. CRISPR/Cas-9 based technology for tailoring of cellulose genes is promising. In silico based gene modulations in cellulase can predict enhanced activity. Magnetic nanoparticle materials are favourable for cellulase recovery strategies. Abstract: The inherent resistance of lignocellulosic biomass makes it impervious for industrially important enzymes such as cellulases to hydrolyze cellulose. Further, the competitive absorption behavior of lignin and hemicellulose for cellulases, due to their electron-rich surfaces augments the inappropriate utilization of these enzymes. Hence, modification of the surface charge of the cellulases to reduce its non-specific binding to lignin and enhance its affinity for cellulose is an urgent necessity. Further, maintaining the stability of cellulases by the preservation of their secondary structures using immobilization techniques will also play an integral role in its industrial production. In silico approaches for increasing the catalytic activity of cellulase enzymes is also significant along with a range of substrate specificity. In addition, enhanced productivity of cellulases by tailoring the related genes through the process of genetic engineering and higher cellulase recovery after saccharification seems to be promising areas for efficient and large-scale enzyme production concepts.