Solid-state anaerobic digestion of sugarcane bagasse at different solid concentrations: Impact of bio augmented cellulolytic bacteria on methane yield and insights on microbial diversity. (November 2021)

• Record Type: Journal Article
• Authors: Arelli, Vijayalakshmi; Mamindlapelli, Naveen Kumar; Juntupally, Sudharshan; Begum, Sameena; Anupoju, Gangagni Rao
• Is Part Of: Bioresource technology. Volume 340(2021)
• Publication Date: 2021-11
• Subjects: Solid state anaerobic digestion -- Methane yield -- Bioaugmentation -- Bagasse -- Metagenomics; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2021.125675
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: Solid State Anaerobic digestion of sugarcane Bagasse. Bioaugmentation by isolated cellulolytic strain of Psuedomonas sp. Methane metabolism majorly driven by acetoclastic methanogens. Total Solid concentration of 40% was found to be optimum. Highest Methane yield of 0.44 L CH4 /(g VS) was obtained due to bioaugmentation. Abstract: This study investigated the impact of the potential cellulose degrading bacteria that could be bioaugmented in the solid-state anaerobic digestion (SSAD) of bagasse to enhance the methane yield. The prospective anaerobic cellulose degrading bacteria was isolated from the soil. SSAD experiments were organized with & without bioaugmentation with a substrate total solid (TS) of 25%, 30%, 40% and 50% at an optimized feed to microorganism (F/M) ratio of 1:1. The maximum yield of 0.44 L CH4 / (g VS added) was obtained from bioaugmented bagasse at a TS of 40% whereas it was 0.34 L CH4 /(g VS added) for non-bioaugmented bagasse. The isolated bacterial strain was identified that belongs species Pseudomonas of Gamma Proteobacteria which exhibited good cellulolytic activity. Metagenomic studies found 90% of archaeal microorganisms affiliated to Methanosaeta, a strict acetoclastic methanogen.