Comprehensive analysis of metabolic changes in rats exposed to acrylamide. (15th October 2021)

• Record Type: Journal Article
• Authors: Zhao, Shiyuan; Zhong, Haitao; Geng, Chunmei; Xue, Hongjia; Wang, Changshui; Sun, Wenxue; Dang, Ruili; Han, Wenxiu; Jiang, Pei
• Is Part Of: Environmental pollution. Volume 287(2021)
• Publication Date: 2021-10-15
• Subjects: Acrylamide -- Biomarker -- Gas chromatography-mass spectrometry -- Metabolomics -- Toxicity mechanism; Pollution -- Periodicals; Pollution -- Environmental aspects -- Periodicals; Environmental Pollution -- Periodicals; Pollution -- Périodiques; Pollution -- Aspect de l'environnement -- Périodiques; Pollution -- Effets physiologiques -- Périodiques; Pollution; Pollution -- Environmental aspects; Periodicals; Electronic journals; 363.73
• DOI: 10.1016/j.envpol.2021.117591
• Languages: English
• ISSNs: 0269-7491

Abstract

Abstract: Acrylamide (ACR) is a widely used environmentally hazardous compound that is known to be neurotoxic, genotoxic, carcinogenic, and reproductive toxicity. It is widely present in soil, water, agents used in chemical industries, and food. It can be distributed to all organs and tissues, and can cause damage to various human systems and those of other animals. Previous metabolomics studies have mainly focused on metabolites in serum and urine, but have lacked comprehensive analysis of major organs and tissues. In the current study, a gas chromatography-massspectrometry method was used to investigate mechanisms underlying organ toxicity, in an effort to identify potentially sensitive biomarkers in the main target tissues of rats after ACR exposure. Male Sprague-Dawley rats were assigned to two groups; a control group and a group treated with 20 mg kg −1 ACR intragastrically for 6 weeks. Metabolite changes in the two groups were statistically analyzed. The respective numbers of altered metabolites in the hippocampus, cortex, kidney, serum, heart, liver, and kidney fat were 21, 21, 17, 5, 15, 14, and 6. There were 14 metabolic pathways related to amino acid, fatty acid, purine, and energy metabolism, revealing that the toxic mechanism of ACR may involve oxidative stress, inflammation, and amino acid metabolism and energy disorders. Graphical abstract: Image 1 Highlights: Acrylamide (ACR) is widely distributed environmental hazardous substance. Comprehensive metabolomic analysis was used to study the effects of ACR on tissues. Multivariate statistics was used to detect metabolite profile changes. ACR exposure caused amino acid, fatty acid, and energy metabolism disorders. ACR exposure caused oxidative stress and inflammation. Abstract : The main focus of the study was metabolic damage and toxicity mechanisms of acrylamide in rat tissues determined via metabolomics methods.