Phytochemicals from the flowers of Prunus persica (L.) Batsch: Anti-adipogenic effect of mandelamide on 3T3-L1 preadipocytes. (1st October 2021)

• Record Type: Journal Article
• Authors: Lee, Dahae; Kim, Ji-Young; Qi, Yutong; Park, Sangsu; Lee, Hye Lim; Yamabe, Noriko; Kim, Hocheol; Jang, Dae Sik; Kang, Ki Sung
• Is Part Of: Bioorganic & medicinal chemistry letters. Volume 49(2021)
• Publication Date: 2021-10-01
• Subjects: Prunus persica (L.) Batsch -- Mandelamide -- Adipocytes -- Adipogenesis; Bioorganic chemistry -- Periodicals; Pharmaceutical chemistry -- Periodicals; 572
• DOI: 10.1016/j.bmcl.2021.128326
• Languages: English
• ISSNs: 0960-894X

Abstract

Graphical abstract: Abstract: Flowers of Prunus persica (L.) Batsch (Rosaceae), known as peach blossoms, have been reported to exert anti-obesity effects by improving hepatic lipid metabolism in obese mice. However, little is known regarding the anti-adipogenic effects of the phenolic compounds isolated from P. persica flowers. This study investigated the inhibitory effects of compounds extracted from P. persica flowers (PPF) on adipogenesis in 3T3-L1 murine preadipocytes using adipogenic differentiation assays. Additionally, we compared the anti-adipogenic effects of the phenolic compounds isolated from PPF, such as prunasin amide (1 ), amygdalin amide (2 ), prunasin acid (3 ), mandelamide (4 ), methyl caffeate (5 ), ferulic acid (6 ), chlorogenic acid (7 ), benzyl α–l -xylpyranosyl-(1 → 6)-β-d -glucopyranoside (8 ), prunin (9 ), naringenin (10 ), nicotiflorin (11 ), astragalin (12 ), afzelin (13 ), and uridine (14 ), on adipogenesis in 3T3-L1 murine preadipocytes. PPF and compounds 4 –7 and 10 significantly inhibited adipogenesis. Among them, mandelamide (4 ) exhibited the maximum inhibitory activity with an IC50 of 36.04 ± 1.82 μM. Additionally, mandelamide downregulated the expression of key adipogenic markers, such as extracellular signal-regulated kinase, c-Jun- N -terminal kinase, P38, CCAAT/enhancer-binding protein α, CCAAT/enhancer-binding protein β, peroxisome proliferator activated receptor γ, and glucocorticoid receptor. These results indicate that mandelamide is an active ingredient of PPF possessing anti-obesity properties.