Decolourisation of textile dye by laccase: Process evaluation and assessment of its degradation bioproducts. (November 2021)

• Record Type: Journal Article
• Authors: Yadav, Ashutosh; Yadav, Pooja; Kumar Singh, Anil; kumar, Vyas; Chintaman Sonawane, Vijay; Markandeya, ; Naresh Bharagava, Ram; Raj, Abhay
• Is Part Of: Bioresource technology. Volume 340(2021)
• Publication Date: 2021-11
• Subjects: Remazol Brilliant Blue R -- Laccase -- Decolourisation -- Molecular docking -- Box- Behnken design; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2021.125591
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: Rapid Remazol Brilliant Blue R dye decolourisation achieved by laccase without redox mediator. Optimization by Box–Behnken design enhanced dye decolourisation (97.18%). Dye degradation has been affirmed by UV–visible spectroscopy and FTIR analysis. Laccase treated dye found relatively less phyto- and cytotoxic. Molecular docking analysis revealed the significant binding affinity between RBBR-laccase. Abstract: Biodegradation of environmentally hazardous synthetic dyes by enzymes has been achieved the highest interest in recent years. In this work, we optimized Remazol Brilliant Blue R (RBBR) dye biodegradation by Arthrographis kalrae derived laccase via the Box-Behnken design (BBD) approach of the surface response methodology (RSM). Optimization of dye decolourisation by one variable at a time (OVAT) approach resulted in optimal dye decolourisation at laccase dose (2 IU mL −1 ), pH (7.0), temperature (35 °C), incubation time (240 min), and initial dye concentration (100 mg L -1 ). The optimized process through BBD enhanced dye decolourisation (97.18%). Fourier Transform Infrared Spectroscopy and UV–Visible Spectrophotometry have proven biodegradation. In addition, in comparison to untreated samples, the laccase-treated dye sample showed relatively less phyto- and cytotoxic effect on Allium cepa L. Extra Precision Glide docking exhibited the binding affinity score of −5.355 kcal mol −1, between laccase-RBBR complex.