Sensitive monitoring mitochondrial peroxynitrite based on a new reaction site and cell imaging by anthracycline-based red emitting fluorescence probe. (November 2021)

• Record Type: Journal Article
• Authors: Li, Minglu; Huang, Yue; Song, Shengmei; Shuang, Shaomin; Wang, Ruibing; Dong, Chuan
• Is Part Of: Dyes and pigments. Volume 195(2021)
• Publication Date: 2021-11
• Subjects: Long-wavelength -- Anthracycline -- Fluorescent probe -- Peroxynitrite -- Co-localization; Dyes and dyeing -- Periodicals; Pigments -- Periodicals; 667.2
• DOI: 10.1016/j.dyepig.2021.109727
• Languages: English
• ISSNs: 0143-7208

Abstract

Abstract: The change in the level of ONOO − is usually indicative of an abnormality in bodily function. Therefore, there is a need to develop highly reliable ONOO − assays to determine its role in the biological environment. Thus, a long-wavelength anthracycline-based fluorescent probe (LAP ) with a new reaction site was presented to detect peroxynitrite (ONOO − ) by one step synthesize between 6-hydroxy-1-tetralone and salicylaldehyde. LAP responded to ONOO − about 8-fold fluorescence changes at 628 nm and the detection limit was 3.7 nM. The ONOO − sensing of LAP with the disruption of the conjugated skeleton was highly specific and could identify ONOO − by naked eyes. LAP was specifically localized to the mitochondria and co-localization results of LAP and MitoTrancker Green showed that the merged fluorescent images were observed with high Pearson's co-localization coefficient 0.92 and 0.90 in SMMC-7721 and RAW264.7 cells, respectively. As a specific and sensitive fluorescence probe, LAP with mitochondria-targetable moiety was ability to detect exogenous ONOO − in SMMC-7721 and RAW264.7 cells. Graphical abstract: Image 1 Highlights: A long-wavelength anthracycline fluorescent probe (LAP ) with a new reaction site was presented to monitor ONOO − . The ONOO − sensing of LAP with the disruption of the conjugated skeleton was highly sensitive and specific. LAP was specifically localized to the mitochondria in SMMC-7721 and RAW264.7 cells. LAP was ability to be specific and sensitive fluorescence probe for detecting exogenous ONOO − in living cells.