Concurrent enhancement of CO2 fixation and productivities of omega-3 fatty acids and astaxanthin in Haematococcus pluvialis culture via calcium-mediated homeoviscous adaptation and biomineralization. (November 2021)

• Record Type: Journal Article
• Authors: Yu, Byung Sun; Sung, Young Joon; Choi, Hong Il; Sirohi, Ranjna; Sim, Sang Jun
• Is Part Of: Bioresource technology. Volume 340(2021)
• Publication Date: 2021-11
• Subjects: Haematococcus pluvialis -- Biomineralization -- Omega-3 fatty acids -- Astaxanthin; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2021.125720
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: ExCA activity and CO2 fixation rate were boosted via Ca 2+ -based biomineralization. The production of ω-3 FA was accelerated via Ca 2+ -induced homeoviscous adaptation. Separated pure H. pluvialis could produce high content of astaxanthin sustainedly. CaCO3 crystal enabled H. pluvialis to cultivate under intensive light conditions. The biomineralization-inspired culture tactic could be well applied to large-scale. Abstract: Haematococcus pluvialis has attracted interest as a bio-platform for producing omega-3 fatty acids (ω-3 FA) and astaxanthin that have a great potential as anti-inflammatory drugs. This study aimed to concurrently enhance the CO2 fixation and the productivities of ω-3 FA and astaxanthin, which have been difficult to achieve because of the dissimilar culture methods for each goal, via calcium-mediated homeoviscous adaptation and biomineralization. As a result of 3 mM of Ca 2+ addition, ω-3 FA content was improved by 31% due to Ca 2+ -induced homeoviscous adaptation. Biomineralization was promoted by the extracellular carbonic anhydrase, which resulted in 46.3% improvement in CO2 fixation. CaCO3 from the biomineralization was beneficially re-used in the H. pluvialis culture and triggered 178- and 522-fold increased biomass productivity and astaxanthin content, respectively, thanks to its anisotropic nature. The Ca 2+ -based productivity enhancement strategy was applied to large-scale culture which resulted improvement in overall bioprocess performance.