Liquid Chromatography–Mass Spectrometry (LC‐MS)‐Based Analysis for Lipophilic Compound Profiling in Plants. Issue 2 (28th April 2020)

• Record Type: Journal Article
• Authors: Lapidot‐Cohen, Taly; Rosental, Leah; Brotman, Yariv
• Editors: Birchler, James; Ecker, Joseph; Martin, Cathie R.; Stitt, Mark; Zhou, Jian-Min
• Other Names: Stacey Gary editorInChief.
• Is Part Of: Current protocols in plant biology. Volume 5:Issue 2(2020)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2020-04-28
• Subjects: fatty acids -- lipid class -- lipidomics -- lipids -- lipid species -- lipophilic compounds -- liquid chromatography–mass spectrometry; Botany -- Laboratory manuals; Plants; Botany; Botany; Laboratory Manual; Periodical; Laboratory manuals; 580.28
• DOI: 10.1002/cppb.20109
• Languages: English
• ISSNs: 2379-8068

Abstract

Abstract: Lipids are fascinating due to their chemical diversity, which is especially vast in the plant kingdom thanks to the high plasticity of the plant biosynthetic machinery. Lipidomic studies aim to simultaneously analyze a large number of lipid compounds of diverse classes in a given sample. The method presented here uses liquid chromatography–mass spectrometry (LC‐MS)‐based lipidomic profiling in a relatively fast, robust, and high‐throughput manner for high‐coverage quantification and annotation of lipophilic compounds. Protocols cover sample preparation, LC‐MS‐based measurement, and data extraction and annotation. An extensive lipid library for triacylglycerols, galactolipids, and phospholipids is provided. The extended profiling described here could be used in a range of applications and is suitable for integration with other omic datasets. © 2020 by John Wiley & Sons, Inc. Basic Protocol 1 : Sample preparation and metabolite extraction Basic Protocol 2 : Liquid chromatography–mass spectrometry (LC‐MS) analysis Basic Protocol 3 : Data extraction, annotation, and quantification