Hydrogen sulfide and rhizobia synergistically regulate nitrogen (N) assimilation and remobilization during N deficiency‐induced senescence in soybean. (18th February 2020)

• Record Type: Journal Article
• Authors: Zhang, Ni‐Na; Zou, Hang; Lin, Xue‐Yuan; Pan, Qing; Zhang, Wei‐Qin; Zhang, Jian‐Hua; Wei, Ge‐Hong; Shangguan, Zhou‐Ping; Chen, Juan
• Is Part Of: Plant, cell and environment. Volume 43:Number 5(2020)
• Page Start: 1130
• Page End: 1147
• Publication Date: 2020-02-18
• Subjects: assimilation -- hydrogen sulfide (H2S) -- nitrogen -- remobilization -- rhizobia -- soybean (Glycine max); Plant physiology -- Periodicals; Plant cells and tissues -- Periodicals; Plant communities -- Periodicals; 581.105
• DOI: 10.1111/pce.13736
• Languages: English
• ISSNs: 0140-7791

Abstract

Abstract: Hydrogen sulfide (H2 S) is emerging as an important signalling molecule that regulates plant growth and abiotic stress responses. However, the roles of H2 S in symbiotic nitrogen (N) assimilation and remobilization have not been characterized. Therefore, we examined how H2 S influences the soybean ( Glycine max )/rhizobia interaction in terms of symbiotic N fixation and mobilization during N deficiency‐induced senescence. H2 S enhanced biomass accumulation and delayed leaf senescence through effects on nodule numbers, leaf chlorophyll contents, leaf N resorption efficiency, and the N contents in different tissues. Moreover, grain numbers and yield were regulated by H2 S and rhizobia, together with N accumulation in the organs, and N use efficiency. The synergistic effects of H2 S and rhizobia were also demonstrated by effects on the enzyme activities, protein abundances, and gene expressions associated with N metabolism, and senescence‐associated genes ( SAG s) expression in soybeans grown under conditions of N deficiency. Taken together, these results show that H2 S and rhizobia accelerate N assimilation and remobilization by regulation of the expression of SAG s during N deficiency‐induced senescence. Thus, H2 S enhances the vegetative and reproductive growth of soybean, presumably through interactions with rhizobia under conditions of N deficiency. Abstract : Hydrogen sulfide (H2 S) is emerging as a signalling molecule implicated in plant growth and abiotic stress response. However, it is unspecified whether H2 S plays a pivotal role in nitrogen (N) assimilation and remobilization in plants. In this study, soybean ( Glycine max ) was chosen as a research object to study the synergistic regulation of H2 S and rhizobia on N absorption and transport. During N deficiency‐induced soybean senescence, the synergistic effect of H2 S and rhizobia regulated the expression of SAG s, including genes involved in protein degradation, nucleic acid degradation, NAC TFs, and receptor‐like protein kinases, affecting the activities, protein abundances, and transcription levels of N metabolism‐related enzymes. However, an increase in the level of N metabolism promoted the assimilation and remobilization of N in soybean by enhancing the N contents in plant tissues and the NRE of the leaves, which ultimately alleviated leaf senescence, and increased the plant biomass and the yield parameters. Therefore, it was demonstrated that H2 S could act synergistically with rhizobia to promote effectively N uptake and transport during N deficiency‐induced soybean senescence, ultimately enhancing the vegetative and reproductive growth of soybean.