Flavonoid constituents of Dobera glabra leaves: amelioration impact against CCl4-induced changes in the genetic materials in male rats. (1st January 2017)

• Record Type: Journal Article
• Authors: Elkhateeb, Ahmed; Abdel Latif, Rasha R.; Marzouk, Mona M.; Hussein, Sameh R.; Kassem, Mona E. S.; Khalil, Wagdy K. B.; El-Ansari, Mohamed A.
• Is Part Of: Pharmaceutical biology. Volume 55:Number 1(2017)
• Page Start: 139
• Page End: 145
• Publication Date: 2017-01-01
• Subjects: ROS -- gene expression -- DNA fragmentation -- glutathione peroxidase; Pharmacognosy -- Periodicals; Materia medica, Vegetable -- Periodicals; 615.321
• DOI: 10.1080/13880209.2016.1230879
• Languages: English
• ISSNs: 1388-0209

Abstract

Abstract: Context: Dobera glabra (Forssk.) Poir (Salvadoraceae) is a highly valued tree with diverse importance as special mineral sourced feed and a folkloric tool for forecasting droughts. However, there are no reports on its phytochemical and biological investigations. Objective: Phytochemical investigation of D. glabra leaves and its protective potential against CCl4 inducing changes in the genetic materials. Materials and methods: D. glabra extract, DGE (70% MeOH/H2 O), was applied to polyamide column chromatography, eluting with MeOH/H2 O of decreasing polarities, followed by preparative chromatographic tools, yielded seven compounds. Three DGE doses (50, 100 and 200 mg/kg bw/d) were administrated for 8 weeks intragastrically to male albino rats prior treated with CCl4 (0.5 mL/kg/bw). The reactive oxygen species (ROS) levels, expression changes of glutamate transporters (GLAST, GLT-1 and SNAT3) mRNA, DNA fragmentation and glutathione peroxidase (GPx) activity were investigated in the liver tissues of these rats. Results: Isorhamnetin-3- O -β-glucopyranoside-7- O -α-rhamnopyranoside, isorhamnetin-3- O -α-rhamnopyranoside-7- O -β-glucopyranoside, kaempferol-3, 7-di- O -α-rhamnopyranoside, isorhamnetin-3- O -β-glucopyranoside, kaempferol-3- O -β-glucopyranoside, isorhamnetin and kaempferol were identified. DGE (200 mg/kg bw) + CCl4 exhibited the most significant reduction in ROS levels and DNA fragmentation with 251.3% and141% compared to 523.1% and 273.2% for CCl4, respectively. Additionally, it increased significantly the mRNA expression of GLAST, GLT-1 and SNAT3 to 2.16-, 1.72- and 2.09-fold, respectively. Also, GPx activity was increased to 4.8 U/mg protein/min compared to CCl4 (1.8 U/mg protein/min). Discussion and conclusion: Flavonoid constituents, antioxidant effect and genotoxic protection activity of D. glabra were first reported. DGE may be valuable in the treatment and hindrance of hepatic oxidative stress and genotoxicity.