Serum metabonomics characterization of liver fibrosis induced by bile duct-ligated in rats and the intervention effects of herb compound 861. Issue 1 (20th January 2019)

• Record Type: Journal Article
• Authors: Liu, Xiao-Jie; Zhang, Cong-En; Yu, Xiao-Hong; Liu, Rui-Xia; Qin, Xue-Mei; Jia, Ji-Dong; Ma, Zhi-Jie
• Is Part Of: Journal of liquid chromatography & related technologies. Volume 42:Issue 1/2(2019)
• Page Start: 31
• Page End: 44
• Publication Date: 2019-01-20
• Subjects: Bile duct ligation -- hepatic fibrosis -- herbal compounds -- LC-MS -- metabolic pathways -- serum metabonomics; Liquid chromatography -- Periodicals; Chromatographic analysis -- Periodicals; Separation (Technology) -- Periodicals; Biotechnology -- Periodicals; Chromatography, Liquid -- Periodicals; 543.8405
• DOI: 10.1080/10826076.2019.1574815
• Languages: English
• ISSNs: 1082-6076

Abstract

Abstract: Liver fibrosis has been increasingly recognized as a cause for high morbidity and mortality of some diseases in humans. Herbal medicines have received great attention due to their low side effects and high safety. Herbal compound 861 (Cpd 861) has been effectively used for treating hepatic fibrosis for long time. Yet, its exact mechanism is still in fancy. Herein, we first established a liver fibrosis model by bile duct ligation (BDL), which led to the toxic accumulation of bile acids in animals, resulting in hepatic fibrosis. A serum metabonomics study on BDL-induced liver fibrosis rats after Cpd 861 treatment was performed using UHPLC-QTOF/MS. Multivariate analysis showed that Cpd 861 significantly reversed the metabolic perturbation induced by BDL to normal state, which is in agreement with the serum biochemical and histopathological findings. 15 metabolites were screened as potential biomarkers. Eight metabolic pathways were recognized as the most relevant pathways, involving dysfunction of amino acids metabolism and synthesis, fatty acid metabolism, phospholipids metabolism, and others. This is the first study to reveal the underlying mechanism of Cpd 861 based on metabonomics, which is complementary to biochemical analysis, and more importantly, a potentially powerful tool to interpret the mechanisms of extremely complex systems. GRAPHICAL ABSTRACT: