Nanoparticle formulation increases Syzygium cumini antioxidant activity in Candida albicans-infected diabetic rats. (1st January 2017)

• Record Type: Journal Article
• Authors: Bitencourt, Paula E. R.; Cargnelutti, Lariane O.; Stein, Carolina S.; Lautenchleger, Raquel; Ferreira, Luana M.; Sangoi, Manuela; Denardi, Laura; Borges, Raphaela M.; Boligon, Aline; Moresco, Rafael N.; Cruz, Letícia; Zanette, Régis A.; Alves, Sydney H.; Moretto, Maria Beatriz
• Is Part Of: Pharmaceutical biology. Volume 55:Number 1(2017)
• Page Start: 1082
• Page End: 1088
• Publication Date: 2017-01-01
• Subjects: Advanced oxidation protein product -- N-acetyl-β-d-glucosaminidase -- TBARS -- streptozotocin -- diabetic complications -- animal models; Pharmacognosy -- Periodicals; Materia medica, Vegetable -- Periodicals; 615.321
• DOI: 10.1080/13880209.2017.1283338
• Languages: English
• ISSNs: 1388-0209

Abstract

Abstract: Context: Syzygium cumini (L.) Skeels (Myrtaceae) is a medicinal plant widely used in folk medicine for the treatment of diabetes mellitus (DM). However, studies on the use of this plant and of nanoparticle formulations against DM-related fungal infections are scarce. Objective: To evaluate the effect of the treatments with aqueous seed extract of S. cumini (ASc) and ASc-loaded polymeric nanoparticles (NPASc) on biochemical parameters in Candida albicans -infected diabetic rats. Materials and methods: Male Wistar rats were divided into eight groups: Control, DM, C. albicans, C. albicans + ASc, C. albicans + NPASc, DM + C. albicans, DM + C. albicans + ASc and DM + C. albicans + NPASc. Rats were daily treated with ASc or NPASc (100 mg/kg) for 21 days. Biochemical parameters in serum and urine, advanced oxidation protein product (AOPP) and TBARS levels in the serum, kidney, liver and pancreas and N -acetyl-β-d -glucosaminidase (NAG) activities in kidney and urine were evaluated. Results: Biochemical and oxidative stress parameters increased in rats with DM and/or candidiasis. NPASc was more effective than ASc in decreasing glucose (56%), cholesterol (33%) and creatinine (51%) levels; serum (16%) and pancreatic (46%) AOPP and renal (48%) TBARS levels when compared with DM + C. albicans group. In C. albicans group, both treatments decreased NAG activity but did not decrease creatinine levels. Conclusions: These data suggest that the use of nanotechnology is able to improve plant extract properties such as antioxidant activity that may be useful in diabetes-related complications.