A novel CgIFNLP receptor involved in regulating ISG expression in oyster Crassostrea gigas. (November 2021)

• Record Type: Journal Article
• Authors: Qiao, Xue; Zong, Yanan; Liu, Zhaoqun; Li, Yuanmei; Wang, Jihan; Wang, Lingling; Song, Linsheng
• Is Part Of: Developmental and comparative immunology. Volume 124(2021)
• Publication Date: 2021-11
• Subjects: Crassostrea gigas -- IFN-Like system -- IFN receptor -- IFN-Stimulated genes; Immunology -- Periodicals; Developmental immunology -- Periodicals; 616.079
• DOI: 10.1016/j.dci.2021.104206
• Languages: English
• ISSNs: 0145-305X

Abstract

Abstract: Interferons (IFNs) are the key coordinators of antiviral immunity by binding to their receptors to orchestrate a complex transcriptional network in vertebrates. Recently, the existence of molluscan IFN-like system has been certified by the identification of important components in IFN system, such as IFN-like protein ( Cg IFNLP) from oyster Crassostrea gigas. In the present study, a novel Cg IFNLP receptor (designed Cg IFNLPR-1) was identified from C. gigas . The open reading frame (ORF) of Cg IFNLPR-1 cDNA was of 1962 bp encoding a peptide of 653 amino acid residues with five fibronectin type III (FNIII) domains and one transmembrane helix region. The mRNA transcripts of Cg IFNLPR-1 were constitutively distributed in all the tested tissues, with the highest level in gonad. After Poly (I:C) stimulation, the mRNA expression of Cg IFNLPR-1 in haemocytes was significantly up-regulated to the highest level at 48 h (4.54-fold of that in control group, p < 0.05). Cg IFNLPR-1 protein was mainly distributed in the cytoplasm and membrane of oyster haemocytes. Cg IFNLP and Cg IFNLPR-1 were able to interact with each other in vitro . After the Cg IFNLPR-1 was knocked down by RNAi, the mRNA expression of IFN-stimulated genes (ISGs), including Cg Mx, Cg Viperin and Cg IFNIP-44, were significantly inhibited after Poly (I:C) stimulation, which was 0.17, 0.31 and 0.53-fold of that in EGFP group, respectively ( p < 0.01). These findings suggested that Cg IFNLPR-1 was a novel Cg IFNLP receptor in the oyster to recognize Cg IFNLP and regulate the expressions of Cg ISGs. Highlights: A novel Cg IFNLP receptor was identified from C. gigas with conserved domain characteristics. Cg IFNLPR-1 mRNA widely distributed in oyster tissues and up-regulated after poly (I:C) stimulation in haemocytes. Cg IFNLPR-1 showed binding capability with Cg IFNLP in vitro. Cg IFNLPR-1 involved in the Cg IFNLP mediated antiviral immunity through regulating ISG expression.