Sequencing and analysis of the complete mitochondrial genome of Gloydius saxatilis (Squamata: Viperidae: Crotalinae). (3rd July 2016)

• Record Type: Journal Article
• Authors: Xu, Chunzhu; Xie, Fei; Liu, Yichen; Zhao, Shuai; Wang, Yongsheng; Ma, Teng; Zhao, Tianqing
• Is Part Of: Mitochondrial DNA. Volume 27:Number 4(2016)
• Page Start: 2361
• Page End: 2362
• Publication Date: 2016-07-03
• Subjects: Control region -- Gloydius saxatilis -- mitogenome -- phylogenetic tree
• DOI: 10.3109/19401736.2015.1025264
• Languages: English
• ISSNs: 2380-2359

Abstract

Abstract: The mitochondrial genome sequence of Gloydius saxatilis is analyzed and presented for the public for the first time. The genome was 17, 218 bp in length and contained 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNA genes and 2 control regions. The overall base composition was A (32.3%), C (28.9%), T (25.8%), and G (13.0%). The base compositions presented clearly the A-C skew, which was most obviously in the protein-coding genes. The extended termination-associated sequence domain, the central conserved domain and the conserved sequence block domain are defined in the mitochondrial genome control region of G. saxatilis . Mitochondrial genomes analyses based on MP, ML, NJ and Bayesian analyses yielded identical phylogenetic trees, indicating a close phylogenetic affinity of the thirteen Crotalinae species. It appeared that no less than two major phyletic lineages were present in Crotalinae. The main clades within the Crotalinae supported are: A clade including the Protobothrops. A clade ( G. brevicaudus, G. ussuriensis, G. intermedius, G. saxatilis) with the Ovophis as the sister taxon to Protobothrops and was supported by bootstrap values of 88%. The four Gloydius species formed a paraphyletic group with the high bootstrap value (100%) in all examinations.