CRISPR-Cas in Streptococcus pyogenes. Issue 4 (3rd April 2019)

• Record Type: Journal Article
• Authors: Le Rhun, Anaïs; Escalera-Maurer, Andrés; Bratovič, Majda; Charpentier, Emmanuelle
• Is Part Of: RNA biology. Volume 16:Issue 4(2019)
• Page Start: 380
• Page End: 389
• Publication Date: 2019-04-03
• Subjects: Streptococcus pyogenes -- mobile genetic elements -- phages -- adaptive immune system -- CRISPR -- Cas9 -- genome editing; RNA -- Periodicals; Molecular biology -- Periodicals; Molecular biology; RNA; Periodicals; 572.8805
• DOI: 10.1080/15476286.2019.1582974
• Languages: English
• ISSNs: 1547-6286

Abstract

ABSTRACT: The discovery and characterization of the prokaryotic CRISPR-Cas immune system has led to a revolution in genome editing and engineering technologies. Despite the fact that most applications emerged after the discovery of the type II-A CRISPR-Cas9 system of Streptococcus pyogenes, its biological importance in this organism has received little attention. Here, we provide a comprehensive overview of the current knowledge about CRISPR-Cas systems from S. pyogenes . We discuss how the interplay between CRISPR-mediated immunity and horizontal gene transfer might have modeled the evolution of this pathogen. We review the current literature about the CRISPR-Cas systems present in S. pyogenes (types I-C and II-A), and describe their distinctive biochemical and functional features. Finally, we summarize the main biotechnological applications that have arisen from the discovery of the CRISPR-Cas9 system in S. pyogenes .