ASSA13-03-42 The Role of CD4+CD25+ Regulatory T Cells in Macrophage-Derived Foam-Cell Formation. (29th April 2013)

• Record Type: Journal Article
• Authors: Lin, Jing; Li, Ming; He, Shaolin; Ma, Xuming; Li, Dazhu
• Is Part Of: Heart. Volume 99(2013)Supplement 1
• Page Start: A27
• Page End: A27
• Publication Date: 2013-04-29
• Subjects: Heart -- Diseases -- Treatment -- Periodicals; Cardiology -- Periodicals; 616.12
• DOI: 10.1136/heartjnl-2013-303992.082
• Languages: English
• ISSNs: 1355-6037

Abstract

Abstract : Background and Objective: Cluster of differentiation (CD)4+ CD25+ regulatory T cells (Tregs) exert a suppressive activity on atherosclerosis, but the underlying mechanism remains unclear. Here, we investigated whether and how Tregs affect macrophages foam-cell formation. Methods and Results: Tregs were isolated by magnetic cell sorting-column and analysed by fl ow cytometry. Macrophages were cultured with or without Tregs in the presence of oxidised LDL (oxLDL) for 48 h to transform foam cells. After co-culture with Tregs, macrophages showed a decrease in lipid accumulation, which was accompanied by a significantly downregulated expression of CD36 and SRA but no obvious difference in ABCA1 expression. Conclusions: Tregs can inhibit the proinflammatory properties of macrophages and steer macrophage differentiation toward an anti-infl amatory cytokine producing phenotype. Mechanistic studies reveal that both cell-to-cell contact and soluble factors are required for Treg-mediated suppression on macrophage foam-cell formation. Cytokines, interleukin-10 (IL-10), and transforming growth factor-beta (TGF-beta) are the key factors for these suppressive functions.