Non‐organ‐specific autoimmunity in adult 47, XXY Klinefelter patients and higher‐grade X‐chromosome aneuploidies. (10th June 2021)

• Record Type: Journal Article
• Authors: Panimolle, Francesca; Tiberti, Claudio; Spaziani, Matteo; Riitano, Gloria; Lucania, Giuseppe; Anzuini, Antonella; Lenzi, Andrea; Gianfrilli, Daniele; Sorice, Maurizio; Radicioni, Antonio F.
• Is Part Of: Clinical and experimental immunology. Volume 205:Number 3(2021)
• Page Start: 316
• Page End: 325
• Publication Date: 2021-06-10
• Subjects: AMA -- ANA -- anti‐DNA -- ASMA -- ENA -- Klinefelter's syndrome -- non‐organ‐specific autoimmunity -- X‐chromosome aneuploidies; Immunopathology -- Periodicals; 616.079
• DOI: 10.1111/cei.13616
• Languages: English
• ISSNs: 0009-9104

Abstract

Abstract: Current literature regarding systemic autoimmune diseases in X‐chromosome aneuploidies is scarce and limited to case reports. Our aim was to evaluate the frequency of anti‐nuclear (ANAs), extractable nuclear (ENA), anti‐double‐stranded DNA (dsDNAs), anti‐smooth muscle (ASMAs) and anti‐mitochondrial (AMAs) antibodies in a large cohort of adults with Klinefelter's syndrome (KS, 47, XXY) and rare higher‐grade sex chromosome aneuploidies (HGAs) for the first time. Sera from 138 X‐chromosome aneuploid patients [124 adult patients with 47, XXY KS and 14 patients with HGA (six children, eight adults)] and 50 age‐matched 46, XY controls were recruited from the Sapienza University of Rome (2007–17) and tested for ANAs, ENAs, anti‐dsDNAs, ASMAs and AMAs. Non‐organ‐specific immunoreactivity was found to be significantly higher in patients with 47, XXY KS (14%) than in the controls (2%, p = 0.002). Among all the antibodies investigated, only ANAs were observed significantly more frequently in patients with 47, XXY KS (12.1%) than in the controls (2%, p = 0.004). No anti‐dsDNA immunoreactivity was found. Stratifying by testosterone replacement therapy (TRT), non‐organ‐specific autoantibody frequencies were higher in TRT‐naive ( p = 0.01) and TRT‐treated groups than in controls. No patients with HGA were found positive for the various autoantibodies. Non‐organ‐specific autoantibodies were significantly present in 47, XXY adult patients. Conversely, HGAs did not appear to be target of non‐organ‐specific immunoreactivity, suggesting that KS and HGAs should be considered as two distinct conditions. The classification and diagnosis of systemic autoimmune diseases is frequently difficult. To support a correct clinical evaluation of KS disease and to prevent eventual secondary irreversible immune‐mediated damages, we highlight the importance of screening for non‐organ‐specific autoimmunity in Klinefelter's syndrome. Abstract : Aim of our study was to evaluate, for the first time, the frequency of anti‐nuclear‐, extractable nuclear‐, anti‐double‐strandedDNA‐, anti‐smooth muscle‐, and anti‐mitochondrial‐antibodies in a large cohort of adults with Klinefelter's syndrome (KS, 47, XXY) and rare higher‐grade sex chromosome aneuploidies (HGAs). Non‐organ–specific autoantibodies were significantly present in 47, XXY adult patients. Conversely, HGAs did not appear to be target of non‐organ–specific immunoreactivity. We highlight the importance of screening for non‐organ–specific autoimmunity in Klinefelter's syndrome to support a correct clinical evaluation of KS disease and to prevent eventual secondary irreversible immune‐mediated damages of systemic autoimmune diseases.