Effects of Low‐Intensity Pulsed Ultrasound on Myelosuppression of Rats Induced by Chemotherapy Drugs With Cell Cycle Specificity. (11th November 2020)

• Record Type: Journal Article
• Authors: Wang, Wei; Chen, Junlin; Luo, Dong; Chen, Jinyun; Xu, Haopeng; Chen, Wenzhi; Wang, Yan
• Is Part Of: Journal of ultrasound in medicine. Volume 40:Number 9(2021)
• Page Start: 1811
• Page End: 1822
• Publication Date: 2020-11-11
• Subjects: docetaxel -- etoposide -- low‐intensity pulsed ultrasound -- myelosuppression; Ultrasonics in medicine -- Periodicals; Ultrasonics; Ultrasonography; Ultrasonics in medicine; Electronic journals; Periodicals; Periodicals; 616.07543
• DOI: 10.1002/jum.15562
• Languages: English
• ISSNs: 0278-4297

Abstract

Abstract : Objectives: To explore the ameliorating effects of low‐intensity pulsed ultrasound (LIPUS) on Sprague Dawley rat myelosuppression induced by cell cycle specificity drugs (docetaxel, mitotic phase sensitive; and etoposide, gap 2 phase sensitive). Methods: Rats were respectively administered docetaxel (100 mg/kg) or etoposide (110 mg/kg) by intraperitoneal injection for 4 consecutive days. Then the rats were divided randomly into a LIPUS group and a non‐LIPUS group. In the LIPUS group, the right femoral metaphysis of rats was treated by LIPUS (acoustic intensity, 200 mW/cm 2 ; frequency, 0.3 MHz; and duty cycle, 20%) for 20 minutes on 7 consecutive days from day 5. The rats of the non‐LIPUS group were treated without ultrasound output. A blood cell count, an enzyme‐linked immunosorbent assay, a real‐time quantitative polymerase chain reaction, and hematoxylin–eosin staining were applied to detect the results. Results: Low‐intensity pulsed ultrasound significantly promoted the counts of bone marrow nucleated cells, white blood cells, immunoglobulin A (IgA), IgG, granulocyte colony‐stimulating factor, stem cell factor, and intercellular cell adhesion molecule 1 and reduced the counts of vascular cell adhesion molecule 1 whether in the docetaxel or etoposide group ( P < .05). Low‐intensity pulsed ultrasound only increased the expression level of IgM in the docetaxel group but decreased the level of interleukin 6 in the etoposide group ( P < .05). Conclusions: Low‐intensity pulsed ultrasound has potential to be a noninvasive treatment for myelosuppression caused by different cell cycle–sensitive chemotherapy drugs.