Adipose tissue senescence is mediated by increased ATP content after a short‐term high‐fat diet exposure. Issue 8 (18th July 2021)

• Record Type: Journal Article
• Authors: Pini, Maria; Czibik, Gabor; Sawaki, Daigo; Mezdari, Zaineb; Braud, Laura; Delmont, Thaïs; Mercedes, Raquel; Martel, Cécile; Buron, Nelly; Marcelin, Geneviève; Borgne‐Sanchez, Annie; Foresti, Roberta; Motterlini, Roberto; Henegar, Corneliu; Derumeaux, Geneviève
• Is Part Of: Aging cell. Volume 20:Issue 8(2021)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2021-07-18
• Subjects: adipose tissue senescence -- ATP -- bioenergetics -- exercise -- obesity -- xanthine oxidase; Cells -- Aging -- Periodicals; 571.8783605
• DOI: 10.1111/acel.13421
• Languages: English
• ISSNs: 1474-9718

Abstract

Abstract: In the context of obesity, senescent cells accumulate in white adipose tissue (WAT). The cellular underpinnings of WAT senescence leading to insulin resistance are not fully elucidated. The objective of the current study was to evaluate the presence of WAT senescence early after initiation of high‐fat diet (HFD, 1–10 weeks) in 5‐month‐old male C57BL/6J mice and the potential role of energy metabolism. We first showed that WAT senescence occurred 2 weeks after HFD as evidenced in whole WAT by increased senescence‐associated ß‐galactosidase activity and cyclin‐dependent kinase inhibitor 1A and 2A expression. WAT senescence affected various WAT cell populations, including preadipocytes, adipose tissue progenitors, and immune cells, together with adipocytes. WAT senescence was associated with higher glycolytic and mitochondrial activity leading to enhanced ATP content in HFD‐derived preadipocytes, as compared with chow diet‐derived preadipocytes. One‐month daily exercise, introduced 5 weeks after HFD, was an effective senostatic strategy, since it reversed WAT cellular senescence, while reducing glycolysis and production of ATP. Interestingly, the beneficial effect of exercise was independent of body weight and fat mass loss. We demonstrated that WAT cellular senescence is one of the earliest events occurring after HFD initiation and is intimately linked to the metabolic state of the cells. Our data uncover a critical role for HFD‐induced elevated ATP as a local danger signal inducing WAT senescence. Exercise exerts beneficial effects on adipose tissue bioenergetics in obesity, reversing cellular senescence, and metabolic abnormalities. Abstract : High‐fat diet (HFD) induced senescence of various white adipose tissue (WAT) cell types as one of the earliest events preceding mitochondrial dysfunction, inflammation, and fibrosis. In WAT of HFD‐fed mice and preadipocytes derived from HFD‐fed mice, ATP levels were increased. The anti‐senescence effects of exercise were partially dependent on purine catabolism as blocking xanthine oxidase activity increased ATP levels and inhibited exercise's rescue effects in subcutaneous WAT.