Effect of pyridoxamine and telmisartan on tubular epithelial cells proliferation and its mechanism. (12th October 2011) - Zhu, Pengli
Abstract : Objective: The purpose of this study was to explore the protective effects of telmisartan and pyridoxamine on tubular epithelial cells (HK-2) in early renal damage. Methods: Cultured HK-2 cells were divided into HK-2 control, angiotensin II (10 −6 mol/l) group (Ang II), telmisartan (10 − 6 mol/l, T) group, pyridoxamine group (1 mmol/l, P1), pyridoxamine group (10 mmol/l, P10), and T (10 − 6 mol/l)+P (10 mmol/l) group (T+P). Methyl thiazolyl tetrazolium (MTT) was used to measure for cell proliferation. Reactive oxygen species (ROS) generation in cellular supernatant was detected by flow cytometry. Real-time quantitative PCR was performed to measure the mRNA expression of transforming growth factor β1 (TGFβ1) and receptor for advanced glycation end-products (RAGE). Results: The OD values of HK-2 were inhibited in Ang II (p<0.05), 10 mmol/l P group and TP group (p<0.01) after both 24 h and 48 h intervention. Synergy of inhibition showed in TP group after 48 h intervention (p <0.01). The level of ROS was reduced in P1, P10 and TP group (p<0.01). There was no statistically significant difference among the three groups (p>0.05). Compared with the Ang II, mRNA expression of RAGE and TGFβ1 were decreased in T, P and TP group (p<0.05). Synergy decreased of TGFβ1 expression was showed in TP group (p<0.01). Conclusion: There is a synergistic effect of inhibiting HK-2 cells proliferation by combined use of telmisartan and pyridoxamine. It may be related to reduce RAGE and TGFβ expression and alleviate oxidative stress on early renal damage.