Effect of Methionine on AMD1 Gene Expression in Prostate Cancer Cells. (18th August 2021)

• Record Type: Journal Article
• Authors: Shen, Shaochen; Zeng, Linhong; Huang, Hongwei
• Is Part Of: Nutrition and cancer. Volume 73:Number 9(2021)
• Page Start: 1804
• Page End: 1815
• Publication Date: 2021-08-18
• Subjects: Cancer -- Periodicals; Nutrition -- Periodicals; Cancer; Nutrition; Periodicals; 616.9940654
• DOI: 10.1080/01635581.2020.1803931
• Languages: English
• ISSNs: 0163-5581

Abstract

Abstract: Objective: To investigate the effect of AMD1 gene expression on prostate cancer cells (PC-3M), explore the mechanism of AMD1 action in cancer cells, and examine the regulation of AMD1 gene expression by methionine (MET). Methods: Quantitative PCR (qPCR) and western blot analysis (WB) approaches were used to detect and measure gene expression. The cell apoptotic rate was determined by flow cytometric (FCM) analysis. Results: qPCR and WB assays showed that both AMD1 gene expression and cell apoptotic rate were associated with MET. Conclusion: MET has a significant regulatory effect on the expression of the AMD1 gene and a certain amount of MET can promote the expression of the AMD1 gene. This provides a health guideline for a low-methionine diet for prostate cancer patients and scientific evidence for prostate cancer prevention.