Trans-Anethole Abrogates Cell Proliferation and Induces Apoptosis through the Mitochondrial-Mediated Pathway in Human Osteosarcoma Cells. (18th August 2021)

• Record Type: Journal Article
• Authors: Pandit, Kritika; Kaur, Sandeep; Kumar, Ajay; Bhardwaj, Renu; Kaur, Satwinderjeet
• Is Part Of: Nutrition and cancer. Volume 73:Number 9(2021)
• Page Start: 1727
• Page End: 1745
• Publication Date: 2021-08-18
• Subjects: Cancer -- Periodicals; Nutrition -- Periodicals; Cancer; Nutrition; Periodicals; 616.9940654
• DOI: 10.1080/01635581.2020.1803927
• Languages: English
• ISSNs: 0163-5581

Abstract

Abstract: trans -Anethole, the major bioactive component of Illicium verum Hook. commonly known as star anise exhibits various pharmacological activities including anti-inflammatory, antimicrobial, insecticidal, and antitumor. Osteosarcoma is an extremely aggressive malignant bone tumor that affects children and young adults and accounts for around 60% of all sarcomas. The study was planned to evaluate the potential of trans -Anethole against Human osteosarcoma cell line MG-63. The antiproliferative activity of trans -Anethole was assessed by MTT assay. trans -Anethole exhibited apoptotic cell death as monitored by confocal/electron microscopy and flow cytometry studies. Modulation of gene expression was studied by Western blot and RT-PCR analysis. The present study revealed that trans -Anethole inhibited osteosarcoma proliferation in a dose-dependent manner with a GI50 value of 60.25 µM and showed pro-apoptotic activity as analyzed by Annexin V-FITC/PI assay. Flow cytometric analysis revealed that trans -Anethole induced cell cycle arrest at the G 0 / G 1 phase with the generation of reactive oxygen species and reduction in mitochondrial membrane potential ( ΔΨm ). Immunoblotting results showed the increased expression of caspase-9/-3, p53, and decreased expression of Bcl-xL suggesting the involvement of the p53 and mitochondrial intrinsic pathway. This work provides a rationale that trans -Anethole might be considered as a promising chemotherapeutic/nutraceutical agent for the management of osteosarcoma. Highlights: trans -Anethole inhibited cell growth and caused G 0 / G 1 arrest in Human osteosarcoma MG-63 cell line. trans -Anethole led to the loss of mitochondrial membrane permeability along with ROS generation. trans -Anethole upregulates the expression of p53, Caspase-9/-3, and downregulate Bcl-xL expression.