Neuroinflammatory inhibitors from Gardneria nutans Siebold & Zuccarini. Issue 44 (9th August 2021)

• Record Type: Journal Article
• Authors: Si, Ying-Ying; Wang, Wei-Wei; Feng, Qing-Mei; Zhao, Zhen-Zhu; Xue, Gui-Min; Sun, Yan-Jun; Feng, Wei-Sheng; Young, Jun-Im; Wang, Xian-Shi
• Is Part Of: RSC advances. Volume 11:Issue 44(2021)
• Page Start: 27085
• Page End: 27091
• Publication Date: 2021-08-09
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/d1ra05204g
• Languages: English
• ISSNs: 2046-2069

Abstract

Abstract : Four new compounds were isolated from G. nutans . Compounds 1–2 are two rare monoterpene indole alkaloids with the glucosyl moiety located at C-12 and represent the first two examples of enantiomer of ajmaline type monoterpene indole alkaloids. Abstract : Two new monoterpene indole alkaloid glycosides nutanoside A–B (1–2 ), two new phenolic glycoside esters nutanester A–B (6–7 ), together with five known compounds (3–5, 8–9 ) were isolated from the ethanol extract of Gardneria nutans Siebold & Zuccarini. Their structures were established on the basis of extensive spectroscopic analysis and TDDFT/ECD calculations. Compounds 1 and 2 are two rare monoterpene indole alkaloids with the glucosyl moiety located at C-12 and represent the first two examples of enantiomer of ajmaline type monoterpene indole alkaloids. Compounds 3, 4 and 6 displayed significant inhibitory effects on NO production in over-activated BV2 microglial cells, with the IC50 values of 2.29, 6.36, and 8.78 μM, respectively. Compounds 1, 5, 7 could significantly inhibit the mRNA expression of inflammatory factors TNF-α and IL-6 induced by LPS in BV2 microglial cells at the effective concentration. Moreover, compound 3 exhibited stronger cytotoxicities against U87 and HCT116 cell lines than taxol with IC50 values of 10.58 and 14.60 μM, respectively.