Kinetics of platelet adhesion to a fibrinogen‐coated surface in whole blood under flow conditions. Issue 9 (4th August 2021)

• Record Type: Journal Article
• Authors: Gabbasov, Zufar A.; Avtaeva, Yuliya N.; Melnikov, Ivan S.; Okhota, Sergey D.; Caprnda, Martin; Mozos, Ioana; Prosecky, Robert; Rodrigo, Luis; Kruzliak, Peter; Zozulya, Nadezhda I.
• Is Part Of: Journal of clinical laboratory analysis. Volume 35:Issue 9(2021)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2021-08-04
• Subjects: fibrinogen‐coated surface -- flow conditions -- platelet adhesion -- recording of a scattered laser light signal from a fibrinogen‐covered surface -- whole blood; Diagnosis, Laboratory -- Periodicals; Medical laboratory technology -- Periodicals; 616
• DOI: 10.1002/jcla.23939
• Languages: English
• ISSNs: 0887-8013

Abstract

Abstract: Aim: To test a novel method of assessment of platelet adhesion to a fibrinogen‐coated surface in whole blood under flow conditions. Methods: We developed a fluidic device that mimics blood flow in vessels. The method of detection of platelet adhesion is based on recording of a scattered laser light signal from a fibrinogen‐covered surface. Testing was performed in platelet‐rich plasma (PRP) and whole blood of healthy volunteers. Control measurements were performed, followed by tests with inhibition of platelet GPIIa/IIIb and GPIb receptors. Then, the same testing sequence was performed in whole blood of persons with autoimmune thrombocytopenia and type 3 von Willebrand disease. Results: The change in intensity of scattered light was 2.7 (2.4; 4.1) times higher in whole blood (0.2 ± 0.08V, n = 7) than in PRP (0.05 ± 0.02 V, n = 7), p < 0.01. The blocking of GP IIb/IIIa receptors decreased the intensity of scattered light to 8.5 (6.5;12)%; the blocking of GPIb receptors decreased it to 34 (23;58)%, p < 0.01. In the whole blood of a person with autoimmune thrombocytopenia, the inhibition of GPIb receptors decreased platelet adhesion, but no effect was observed in type 3 von Willebrand disease. Inhibition of platelet GPIIb/IIIa receptors alone or combined inhibition of GPIb and GPIIb/IIIa receptors resulted in almost total suppression of adhesion in both cases. Conclusion: Our system effectively registers platelet adhesion to a fibrinogen‐coated surface under controlled‐flow conditions and may successfully be applied to the investigation of platelet adhesion kinetics. Abstract : The laser optical system for recording of platelet adhesion to protein‐coated surfaces under flow conditions. The illustration of the flow chamber with the flat glass surface coated with a monolayer of cell‐adhesive molecules. The glass surface was coated with fibrinogen. The reflected light evanesces into the blood sample beyond the reflection zone to a depth of approximately 400–600 nm. Platelets or other blood cells in the flow do not interact with this laser radiation, which evanesces to such a small depth. The laser light can interact with platelets only when they are strongly attached to or spread onto the glass surface.