Ac106/107 affects production of infectious progeny BV by regulating transcription of late viral genes and host cell energy metabolism. Issue 10 (2nd July 2021)

• Record Type: Journal Article
• Authors: Wang, Jingli; Li, Ke; Fu, Yuejun
• Is Part Of: Pest management science. Volume 77:Issue 10(2021)
• Page Start: 4758
• Page End: 4769
• Publication Date: 2021-07-02
• Subjects: Autograph californica multicapsid nucleopolyhedrovirus (AcMNPV) -- Spodoptera frugiperda 9 cell -- Ac106/107 -- insect resistance gene; Pests -- Control -- Periodicals; Pesticides -- Periodicals; 632.9
• DOI: 10.1002/ps.6520
• Languages: English
• ISSNs: 1526-498X

Abstract

Abstract: BACKGROUND: AcMNPV is a model organism of baculovirus, and Spodoptera frugiperda is one of its hosts. Disclosing the role of ac106/107 in AcMNPV infecting Spodoptera frugiperda 9 (Sf9) cells is of great significance for modifying AcMNPV as a microbial insecticide. This work constructed recombinant baculovirus that knocking out, repairment and overexpression of ac106/107 and explored the effects of Ac106/107 on the proliferation of progeny viruses. Moreover, the potential mechanism and targets of ac106/107 were further revealed. RESULTS: First, compared with the Bacmid‐EGFP transfection group, the progeny virus does not proliferate after knocking out of ac106/107, and the proliferation ability increases by 14.5% at 72 h post transfection (h p.t.) when overexpression of ac106/107 . However, knockout, repairment and overexpression of ac106/107 have no effect on viral DNA replication. Secondly, Ac106/107‐EGFP was located in the cytoplasm and nucleus. Transcription level of late viral genes and viral RNA polymerase subunit genes in the Bacmid ac106/107 KO ‐EGFP transfection group and Bacmid‐Ac106/107‐EGFP transfection group was reduced and increased, respectively. Thirdly, AcMNPV would increase the glucose utilization and lactate consumption of the host Sf9 cells, and Bacmid ac106/107 KO ‐EGFP transfection group had lower glucose consumption and lactic acid accumulation than Bacmid‐EGFP, Bacmid ac106/107 KO ‐Ac106/107(rep)‐EGFP and Bacmid‐Ac106/107‐EGFP transfection groups. CONCLUSION: Ac106/107 can enter the nucleus and affect transcription of viral RNA polymerase subunit genes, which in turn affects the transcription of late genes, and ultimately affects virus proliferation and energy metabolism in host cells. © 2021 Society of Chemical Industry. Abstract : Budding AcMNPV entered the cell under the endocytosis mediated by clathrin and was encapsulated in the endocytosis. In the acidic environment of the endosome, the viral envelope and endosome membrane fused, and the nucleocapsid was released. Then the nucleocapsid was pushed into the nucleus by the comet‐like tail formed by actin. The virus performs DNA replication and transcription in the VS region, and is translated into protein in the cytoplasm. Ac106/107 enters the nucleus to perform its functions. This study indicated that Ac106/107 would not affect DNA replication, but would promote 38k, vp39 and gp64 transcription by promoting viral RNA polymerase synthesis and thus promote viral nucleocapsid assembly and envelope fusion. The blue arrow in the figure indicates the virus replication process, and the red arrow indicates the promotion.