Rapid Capture of Cancer Extracellular Vesicles by Lipid Patch Microarrays. Issue 35 (26th July 2021)

• Record Type: Journal Article
• Authors: Liu, Hui‐Yu; Kumar, Ravi; Zhong, Chunting; Gorji, Saleh; Paniushkina, Liliia; Masood, Ramsha; Wittel, Uwe A.; Fuchs, Harald; Nazarenko, Irina; Hirtz, Michael
• Is Part Of: Advanced materials. Volume 33:Issue 35(2021)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2021-07-26
• Subjects: breast cancer -- dip‐pen nanolithography -- extracellular vesicles -- scanning probe lithography -- supported lipid bilayers; Materials -- Periodicals; Chemical vapor deposition -- Periodicals; 620.11
• DOI: 10.1002/adma.202008493
• Languages: English
• ISSNs: 0935-9648

Abstract

Abstract: Extracellular vesicles (EVs) contain various bioactive molecules such as DNA, RNA, and proteins, and play a key role in the regulation of cancer progression. Furthermore, cancer‐associated EVs carry specific biomarkers and can be used in liquid biopsy for cancer detection. However, it is still technically challenging and time consuming to detect or isolate cancer‐associated EVs from complex biofluids (e.g., blood). Here, a novel EV‐capture strategy based on dip‐pen nanolithography generated microarrays of supported lipid membranes is presented. These arrays carry specific antibodies recognizing EV‐ and cancer‐specific surface biomarkers, enabling highly selective and efficient capture. Importantly, it is shown that the nucleic acid cargo of captured EVs is retained on the lipid array, providing the potential for downstream analysis. Finally, the feasibility of EV capture from patient sera is demonstrated. The demonstrated platform offers rapid capture, high specificity, and sensitivity, with only a small need in analyte volume and without additional purification steps. The platform is applied in context of cancer‐associated EVs, but it can easily be adapted to other diagnostic EV targets by use of corresponding antibodies. Abstract : Extracellular vesicles have great prospects as diagnostic and therapeutic tools. However, their isolation still poses challenges. A novel supported lipid‐membrane‐based platform is introduced for rapid capture, with high specificity and sensitivity, only a small needed analyte volume, and without additional purification steps. The platform is flexible and can be applied to many targets by corresponding antibodies.