MiR-148-1-5p modulates NF-κB signaling pathway by targeting IRAK1 in miiuy croaker (Miichthys miiuy). (December 2021)

• Record Type: Journal Article
• Authors: Chang, Renjie; Zheng, Weiwei; Luo, Qiang; Liu, Guiliang; Xu, Tianjun; Sun, Yuena
• Is Part Of: Developmental and comparative immunology. Volume 125(2021)
• Publication Date: 2021-12
• Subjects: microRNA -- IRAK1 -- Gene regulation -- Innate immune -- NF-κB; Immunology -- Periodicals; Developmental immunology -- Periodicals; 616.079
• DOI: 10.1016/j.dci.2021.104229
• Languages: English
• ISSNs: 0145-305X

Abstract

Abstract: microRNAs (miRNAs), a crucial class of small non-coding RNA species, have been extensively studied as key molecular in immune regulation in the past decades. Here, we discover a new miRNA miR-148-1-5p and we elaborate that miR-148-1-5p functions as a negative regulator to participate in innate immune responses. In this article, it has been researched that the regulation effect of miR-148-1-5p to the nuclear factor kappaB (NF-κB) signaling pathway by targeting IRAK1 in miiuy croaker. First, through bioinformatics software to predict the potential targets of miR-148-1-5p, we found that IRAK1 had a base complementary region with indicated miRNA. Next, the dual-luciferase assays revealed that overexpression of miR-148-1-5p mimics and pre-miR-148 plasmid could significantly inhibit the luciferase activity of wild-type IRAK1-3′UTR. However, miR-148-1-5p inhibitors attenuated the inhibition caused by miR-148-1-5p. In addition, we also confirmed that miR-148-1-5p could suppress the expression of IRAK1 at mRNA level. Collectively, the regulations of miR-148-1-5p to NF-κB signaling pathways via targeting the IRAK1 gene was studied in miiuy croaker, which provided new information to enrich the immune regulation network of miRNA in teleost fish. Highlights: miR-148-1-5p targets miiuy croaker IRAK1. miR-148-1-5p inhibits the expression of IRAK1 at mRNA level. miR-148-1-5p inhibits NF-κB signaling pathway by targeting IRAK1.