Hemocyte migration and expression of four Sox genes during wound healing in Pacific abalone, Haliotis discus hannai. Issue 117 (October 2021)

• Record Type: Journal Article
• Authors: Furukawa, Fumiya; Doshimo, Yumi; Sodeyama, Gin; Adachi, Kenta; Mori, Kazuma; Mori, Yuichi; Inadama, Ryota; Koyama, Mugen; Funayama, Shohei; Oda, Takuji; Furukawa, Suehiro; Moriyama, Shunsuke; Kimura, Satoshi; Kaneko, Toyoji; Okumura, Sei-ichi
• Is Part Of: Fish & shellfish immunology. Issue 117(2021)
• Page Start: 24
• Page End: 35
• Publication Date: 2021-10
• Subjects: Mollusc -- GastropodWound healing -- Cell migration -- Sox; PCNA proliferating cell nuclear antigen -- Sox SRY-related HMG box -- SRY sex-determining region Y -- HMG box high mobility group box; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2021.07.011
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: In molluscs, migration of hemocytes and epithelial cells is believed to play central roles in wound healing. Here, we assessed cellular and molecular mechanisms of wound healing in Pacific abalone, a marine gastropod. Light and electron microscopy in the wounds showed early accumulation of putative hemocytes, collagen deposition by fibroblasts, and further coverage of this tissue by migration of adjacent epithelial cells. Cell labelling technique allowed us to track hemocytes, which migrated to wound surface within 24 h. The migrated cells first expressed PCNA and SoxF weakly, and then the epithelial cells expressed abundant PCNA and SoxB1, SoxB2, and SoxC . These findings imply that abalone SoxF is involved in hemocyte migration or their differentiation into fibroblasts, and suggest that the migrated epithelia acquire stem cell-like property and undergo active proliferation. This study is the first to show direct evidence of hemocyte migration to wounds and expression of Sox genes in molluscan wound healing. Highlights: Wound healing in epipodium involved migration of hemocytes and adjacent epithelia. Fluorescent-labelled hemocytes were tracked in vivo to show migration to the wound. SoxF was specifically expressed in hemocytes migrating to the wound. The migrated epithelia expressed SoxB1, SoxB2, SoxC, and PCNA.