Enantioselective supercritical fluid chromatography–tandem mass spectrometry method for simultaneous estimation of risperidone and its 9-hydroxyl metabolites in rat plasma. (December 2017)

• Record Type: Journal Article
• Authors: Prasad, Thatipamula R; Joseph, Siji; Kole, Prashant; Kumar, Anoop; Subramanian, Murali; Rajagopalan, Sudha; KR, Prabhakar
• Is Part Of: Bioanalysis. Volume 9:Number 22(2017)
• Page Start: 1739
• Page End: 1750
• Publication Date: 2017-12
• Subjects: 9-hydroxyrisperidone -- chiral metabolites -- pharmacokinetics -- risperidone -- SFC–MS/MS -- supercritical fluid chromatography; Drugs -- Analysis -- Periodicals; 615.19005
• DOI: 10.4155/bio-2017-0168
• Languages: English
• ISSNs: 1757-6180

Abstract

Aim: Objective of the current work was to develop a 'green chemistry' compliant selective and sensitive supercritical fluid chromatography–tandem mass spectrometry method for simultaneous estimation of risperidone (RIS) and its chiral metabolites in rat plasma.Methodology & results: Agilent 1260 Infinity analytical supercritical fluid chromatography system resolved RIS and its chiral metabolites within runtime of 6 min using a gradient chromatography method. Using a simple protein precipitation sample preparation followed by mass spectrometric detection achieved a sensitivity of 0.92 nM (lower limit of quantification). With linearity over four log units (0.91–7500 nM), the method was found to be selective, accurate, precise and robust.Conclusion: The method was validated and was successfully applied for simultaneous estimation of RIS and 9-hydroxyrisperidone metabolites (R & S individually) after intravenous and per oral administration to rats.