Molecular mechanisms underlying responses of the Antarctic coral Malacobelemnon daytoni to ocean acidification. (August 2021)

• Record Type: Journal Article
• Authors: Servetto, N.; de Aranzamendi, M.C.; Bettencourt, R.; Held, C.; Abele, D.; Movilla, J.; González, G.; Bustos, D.M.; Sahade, R.
• Is Part Of: Marine environmental research. Volume 170(2021)
• Publication Date: 2021-08
• Subjects: Ocean acidification -- Coral Malacobelemnon daytoni -- Antarctica -- Gene expression; Marine pollution -- Environmental aspects -- Periodicals; Marine ecology -- Periodicals; Mer -- Pollution -- Aspect de l'environnement -- Périodiques; Écologie marine -- Périodiques; Electronic journals; 577.705
• DOI: 10.1016/j.marenvres.2021.105430
• Languages: English
• ISSNs: 0141-1136

Abstract

Abstract: Benthic organisms of the Southern Ocean are particularly vulnerable to ocean acidification (OA), as they inhabit cold waters where calcite-aragonite saturation states are naturally low. OA most strongly affects animals with calcium carbonate skeletons or shells, such as corals and mollusks. We exposed the abundant cold-water coral Malacobelemnon daytoni from an Antarctic fjord to low pH seawater (LpH) (7.68 ± 0.17) to test its physiological responses to OA, at the level of gene expression (RT-PCR) and enzyme activity. Corals were exposed in short- (3 days) and long-term (54 days) experiments to two pCO2 conditions (ambient and elevated pCO2 equaling RCP 8.5, IPCC 2019, approximately 372.53 and 956.78 μatm, respectively). Of the eleven genes studied through RT-PCR, six were significantly upregulated compared with control in the short-term in the LpH condition, including the antioxidant enzyme superoxide dismutase (SOD), Heat Shock Protein 70 (HSP70), Toll-like receptor (TLR), galaxin and ferritin. After long-term exposure to low pH conditions, RT-PCR analysis showed seven genes were upregulated. These include the mannose-binding C-Lectin and HSP90. Also, the expression of TLR and galaxin, among others, continued to be upregulated after long-term exposure to LpH. Expression of carbonic anhydrase (CA), a key enzyme involved in calcification, was also significantly upregulated after long-term exposure. Our results indicated that, after two months, M. daytoni is not acclimatized to this experimental LpH condition. Gene expression profiles revealed molecular impacts that were not evident at the enzyme activity level. Consequently, understanding the molecular mechanisms behind the physiological processes in the response of a coral to LpH is critical to understanding the ability of polar species to cope with future environmental changes. Approaches integrating molecular tools into Antarctic ecological and/or conservation research make an essential contribution given the current ongoing OA processes. Highlights: Gene expression profiles of Malacobelemnon daytoni revealed an important number of transcripts upregulated in response to Low pH. The biomineralization is not acclimated after two months in the Low pH condition. Malacobelemnon daytoni is working extra hard at the molecular level to maintain the same enzyme activity levels.