Sensitive identification of prostate‐specific antigen by iron oxide nanoparticle antibody conjugates on the gap‐finger electrode surface. (13th September 2020)

• Record Type: Journal Article
• Authors: Zhang, Wei; Li, Kai; Guo, Jingyang; Ma, Tao; Wang, Dongqing; Shi, Sumei; Gopinath, Subash C. B.; Gu, Deqiang
• Is Part Of: Biotechnology and applied biochemistry. Volume 68:Number 4(2021)
• Page Start: 896
• Page End: 901
• Publication Date: 2020-09-13
• Subjects: biomarker -- interdigitated electrode -- metallic nanoparticle -- prostate cancer; Biotechnology -- Periodicals; Biochemical engineering -- Periodicals; Biochemistry -- Periodicals; Biochemistry -- Periodicals; Genetic Techniques -- Periodicals; Microbiological Techniques -- Periodicals; 660.6
• DOI: 10.1002/bab.2012
• Languages: English
• ISSNs: 0885-4513

Abstract

Abstract: Researches have proved that increasing level of prostate‐specific antigen (PSA) is an indicator for the progression of prostate cancer. The present study was focused to determine the PSA level by using anti‐PSA antibody conjugated iron oxide nanoparticles, as the probe immobilized on the gap‐fingered electrode sensing surface. The detection limit and sensitivity were found at the level of 1.9 pg/mL on the linear regression curve ( y = 1.6939 x − 0.5671; R ² = 0.9878). A dose‐dependent liner range was found from 1.9 until 60 pg/mL. Further, PSA was spiked in human serum and did not affect the interaction of PSA and its antibody. This method of detection quantifies the level of PSA, which helps to diagnose prostate cancer at its earlier stage. Abstract : Identification of prostate specific antigen by iron oxide nanoparticle antibody conjugates.