N-Heterocyclic carbenes meet toll-like receptors. Issue 68 (10th August 2021)

• Record Type: Journal Article
• Authors: Singh, Ishwar; Lee, Dianne S.; Huang, Shuaishuai; Bhattacharjee, Hridaynath; Xu, Wei; McLeod, Jennifer F.; Crudden, Cathleen M.; She, Zhe
• Is Part Of: Chemical communications. Volume 57:Issue 68(2021)
• Page Start: 8421
• Page End: 8424
• Publication Date: 2021-08-10
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/d1cc03030b
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : First NHC-TLR biosensor. Modular construction. Whole-cell bacteria detection. Abstract : Combining the stability of the N-heterocyclic carbenes (NHCs) and broad-spectrum recognition of toll-like receptor (TLR) proteins, we report new electrochemical biosensors for bacteria detection. Instead of traditional thiol–gold chemistry, newly synthesized NHCs are employed as the linker molecules to immobilize TLR bio-recognition elements on gold electrodes. Our proof-of-concept methodology includes testing the fidelity of TLR-based electrochemical sensors with NHC linkers. The performance of the biosensors is demonstrated using whole-cell bacterial cultures.