MiR-219-5p targets TBXT and inhibits breast cancer cell EMT and cell migration and invasion. Issue 8 (27th August 2021)

• Record Type: Journal Article
• Authors: Ye, Qin; Wang, Xing; Yuan, Mei; Cui, Shuaishuai; Chen, Yuanyuan; Hu, Zhaodi; Liu, Dandan; Han, Conghui; Li, Bibo; Chen, Dahu
• Is Part Of: Bioscience reports. Volume 41:Issue 8(2021)
• Publication Date: 2021-08-27
• Subjects: cell invasion -- cell migration -- EMT -- miRNA -- TBXT; Molecular biology -- Periodicals; Cytology -- Periodicals; 572.8
• DOI: 10.1042/BSR20210318
• Languages: English
• ISSNs: 0144-8463

Abstract

Abstract: miR-219-5p has been reported to act as either a tumor suppressor or a tumor promoter in different cancers by targeting different genes. In the present study, we demonstrated that miR-219-5p negatively regulated the expression of TBXT, a known epithelial–mesenchymal transition (EMT) inducer, by directly binding to TBXT 3′-untranslated region. As a result of its inhibition on TBXT expression, miR-219-5p suppressed EMT and cell migration and invasion in breast cancer cells. The re-introduction of TBXT in miR-219-5p overexpressing cells decreased the inhibitory effects of miR-219 on EMT and cell migration and invasion. Moreover, miR-219-5p decreased breast cancer stem cell (CSC) marker genes expression and reduced the mammosphere forming capability of cells. Overall, our study highlighted that TBXT is a novel target of miR-219-5p. By suppressing TBXT, miR-219-5p plays an important role in EMT and cell migration and invasion of breast cancer cells.