Omega-3 polyunsaturated fatty acids ameliorated inflammatory response of mammary epithelial cells and mammary gland induced by lipopolysaccharide. (9th August 2021)

• Record Type: Journal Article
• Authors: Feng, Jiaxin; Wang, Qianwen; Yang, Wei; Liu, Jun; Gao, Ming-Qing
• Is Part Of: Acta biochimica et biophysica Sinica. Volume 53:Number 9(2021)
• Page Start: 1142
• Page End: 1153
• Publication Date: 2021-08-09
• Subjects: omega-3 polyunsaturated fatty acids -- lipopolysaccharide -- inflammation -- NF-κB signaling pathways -- intestinal microbiota; Biochemistry -- Periodicals; Biophysics -- Periodicals; 572.05
• DOI: 10.1093/abbs/gmab100
• Languages: English
• ISSNs: 1672-9145

Abstract

Abstract: Omega-3 polyunsaturated fatty acids (n-3 PUFAs), essential fatty acids for humans and animals, have been reported to play a beneficial role in a variety of inflammatory diseases. In this study, we investigated the inhibitory effects and potential molecular mechanisms of n-3 PUFAs on the inflammatory response in lipopolysaccharide (LPS)-stimulated mammary alveolar cell line (MAC-T). Results showed that n-3 PUFAs could abate LPS-induced secretions of tumor necrosis factor-α, interleukin (IL)-6 and IL-1β in MAC-T cells through the nuclear transcription factor kappa B (NF-κB) signal pathway. Meanwhile, n-3 PUFA intervention attenuated histopathologic changes of mammary glands, the white blood cell number decrease, and the alkaline phosphatase level decrease in the serum of mice challenged by LPS. Furthermore, n-3 PUFA intervention improved the ecological structure of the flora in terms of the structural disorder of the non-significant dominant flora induced by LPS in mice. Collectively, both in vitro and in vivo experiments revealed that n-3 PUFAs have a positive effect on LPS-induced inflammatory response, which was possibly mediated by the NF-κB signaling pathway and the intestinal microbiota.