Psychrosphaera ytuae sp. nov., isolated from the deep-sea cold seep sediment of South China Sea. Issue 8 (25th August 2021)

• Record Type: Journal Article
• Authors: Xu, Xiu-dan; Zhang, Jian; Sun, Qing-lei; Sun, Yuan-yuan; Tang, Yong-zheng
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 71:Issue 8(2021)
• Publication Date: 2021-08-25
• Subjects: Alphaproteobacteria -- cold seep -- Psychrosphaera -- sediment; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.004983
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : In this study, we report a Gram-stain-negative, rod-shaped, non-pigmented, motile and aerobic bacterium named strain MTZ26 T, which was isolated from deep-sea sediment sampled at a cold seep in the South China Sea. Growth of strain MTZ26 T occurred at 4–40 °C (optimum, 25–30 °C), pH 6.0–10.0 (optimum, 7.0–8.0) and with 1.0–11.0 % (w/v) NaCl (optimum, 6.0–8.0 %). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain MTZ26 T belonged to the genus Psychrosphaera and was closely related to Psychrosphaera aestuarii PSC101 T (97.5 % sequence similarity) and Psychrosphaera haliotis KDW4 T (97.5 %). Genomic analysis indicated that strain MTZ26 T contains a circular chromosome of 3 331 814 bp with G+C content of 42.2 mol%. The predominant respiratory quinone of MTZ26 T was ubiquinone-8. The polar lipids of MTZ26 T contained phosphatidylglycerol, phosphatidylethanolamine, one unidentified aminophospholipid and one unidentified phospholipid. The major fatty acids of strain MTZ26 T contained C15:0, C16:0, C17:0, C17 : 1 ω8 c, C10 : 0 3-OH, C11 : 0 3-OH, C15 : 1 ω8 c and summed feature 8 (C18 : 1 ω7 c or/and C18 : 1 ω6 c ). Results of phylogenetic, physiological, biochemical and morphological analyses suggested that strain MTZ26 T represents a novel species of the genus Psychrosphaera, and the name Psychrosphaera ytuae sp. nov. is proposed with the type strain MTZ26 T (=MCCC 1K05568 T =JCM 34321 T ).