Performance comparison of micro-neutralization assays based on surrogate SARS-CoV-2 and WT SARS-CoV-2 in assessing virus-neutralizing capacity of anti-SARS-CoV-2 antibodies. Issue 8 (26th August 2021)

• Record Type: Journal Article
• Authors: Sekirov, Inna; Petric, Martin; Carruthers, Erin; Lawrence, David; Pidduck, Tamara; Kustra, Jesse; Laley, Jonathan; Lee, Min-Kuang; Chahil, Navdeep; Mak, Annie; Levett, Paul N.; Mendoza, Emelissa; Wood, Heidi; Drebot, Mike; Krajden, Mel; Morshed, Muhammad
• Is Part Of: Access microbiology. Volume 3:Issue 8(2021)
• Publication Date: 2021-08-26
• Subjects: SARS CoV-2 -- COVID-19 -- PRNT -- enzyme immunoassay -- microneutralization -- neutralization -- serology; Microbiology -- Periodicals; 579
• DOI: 10.1099/acmi.0.000257
• Languages: English
• ISSNs: 2516-8290

Abstract

Abstract : We compared neutralization assays using either the wild-type severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus or surrogate neutralization markers, using characterized sera. We found the results of the neutralization assays 75 % concordant overall and 80 % concordant for samples with high antibody levels. This demonstrates that commercial surrogate SARS-CoV-2 assays offer the potential to assess anti-SARS-CoV-2 antibodies' neutralizing capacity outside CL-3 laboratory containment.