The fecal microbiome and rotavirus vaccine immunogenicity in rural Zimbabwean infants. Issue 38 (7th September 2021)

• Record Type: Journal Article
• Authors: Robertson, Ruairi C.; Church, James A.; Edens, Thaddeus J.; Mutasa, Kuda; Min Geum, Hyun; Baharmand, Iman; Gill, Sandeep K.; Ntozini, Robert; Chasekwa, Bernard; Carr, Lynnea; Majo, Florence D.; Kirkpatrick, Beth D.; Lee, Benjamin; Moulton, Lawrence H.; Humphrey, Jean H.; Prendergast, Andrew J.; Manges, Amee R.
• Is Part Of: Vaccine. Volume 39:Issue 38(2021)
• Page Start: 5391
• Page End: 5400
• Publication Date: 2021-09-07
• Subjects: Microbiota -- Microbiome -- Rotavirus -- Shotgun metagenomics -- Oral rotavirus vaccine; Vaccines -- Periodicals; 615.372
• DOI: 10.1016/j.vaccine.2021.07.076
• Languages: English
• ISSNs: 0264-410X

Abstract

Abstract: Background: Oral rotavirus vaccine (RVV) immunogenicity is considerably lower in low- versus high-income populations; however, the mechanisms underlying this remain unclear. Previous evidence suggests that the gut microbiota may contribute to differences in oral vaccine efficacy. Methods: We performed whole metagenome shotgun sequencing on stool samples and measured anti-rotavirus immunoglobulin A in plasma samples from a subset of infants enrolled in a cluster randomized 2 × 2 factorial trial of improved water, sanitation and hygiene and infant feeding in rural Zimbabwe (SHINE trial: NCT01824940). We examined taxonomic microbiome composition and functional metagenome features using random forest models, differential abundance testing and regression analyses to explored associations with RVV immunogenicity. Results: Among 158 infants with stool samples and anti-rotavirus IgA titres, 34 were RVV seroconverters. The median age at stool collection was 43 days (IQR: 35–68), corresponding to a median of 4 days before the first RVV dose. The infant microbiome was dominated by Bifidobacterium longum . The gut microbiome differed significantly between early (≤42 days) and later samples (>42 days) however, we observed no meaningful differences in alpha diversity, beta diversity, species composition or functional metagenomic features by RVV seroconversion status. Bacteroides thetaiotaomicron was the only species associated with anti-rotavirus IgA titre. Random forest models poorly classified seroconversion status by both composition and functional microbiome variables. Conclusions: RVV immunogenicity is low in this rural Zimbabwean setting, however it was not associated with the composition or function of the early-life gut microbiome in this study. Further research is warranted to examine the mechanisms of poor oral RVV efficacy in low-income countries.