Epithelial-to-mesenchymal transition contributes to the downregulation of progesterone receptor expression in endometriosis lesions. Issue 212 (September 2021)

• Record Type: Journal Article
• Authors: Ma, Lijuan; Andrieu, Thomas; McKinnon, Brett; Duempelmann, Lea; Peng, Ren-Wang; Wotzkow, Carlos; Müller, Christoph; Mueller, Michael D.
• Is Part Of: Journal of steroid biochemistry and molecular biology. Issue 212(2021)
• Publication Date: 2021-09
• Subjects: Progesterone receptor -- EMT -- Endometriosis -- SNAI -- Progesterone resistance; Steroid hormones -- Periodicals; Biochemistry -- Periodicals; Hormones -- Periodicals; Molecular Biology -- Periodicals; Hormones stéroïdes -- Périodiques; Steroid hormones; Periodicals; 572.579
• DOI: 10.1016/j.jsbmb.2021.105943
• Languages: English
• ISSNs: 0960-0760

Abstract

Highlights: Diverse states of Epithelial-to-Mesenchymal Transition (EMT) are present in endometriotic lesions. Presence of EMT is concurrent with low PR expression in endometriotic lesions. PR expression negatively correlates with SNAI1/2 expression in endometriotic lesions. EMT contributes to the downregulation of PR via SNAI1/2 in endometriosis. Abstract: Endometriosis is a common, estrogen-dependent disease, in which endometrial tissue grows in the peritoneal cavity. These lesions often express low levels of progesterone receptors (PR), which potentially play an important role in the insufficient response to progestin treatment. Here, we uncover an interconnection between the downregulated PR expression and the epithelial-to-mesenchymal transition (EMT) in endometriotic lesions. The majority of ectopic epithelial glands (93.1 %, n = 67/72) display heterogeneous states of EMT by immunohistochemistry staining. Interestingly, low PR expression associated with high N-cadherin expression, a hallmark of EMT. In order to gain mechanistic insights, we performed in vitro functional assays with the endometriotic epithelial cell lines EM'osis and 12Z. TGF-β-induced EMT, marked by elevations of CDH2 and SNAI1/2, led to a significant downregulation of PR gene expression in both cell lines. In contrast, silencing of SNAI1 in EM'osis and of SNAI1 plus SNAI2 in 12Z elevated PR gene expression significantly. We found that not only in vitro, but also in the epithelial component of endometriotic lesions strong expression of SNAI1/2 concurred with weak expression of PR. In summary, these results suggested the negative correlation association of the heterogeneous states of EMT and suppressed PR expression in endometriotic lesions. Our functional assays indicate that EMT contributes to the downregulation of PR expression via the upregulation of EMT-TFs, like SNAI1 and SNAI2, which may ultimately lead to progesterone resistance.