Orthogonal processing strategies to create "phage-free" whey – Membrane filtration followed by thermal or ultraviolet C treatment for the reduction of Lactococcus lactis bacteriophages. (November 2021)

• Record Type: Journal Article
• Authors: Michel, Christina; Samtlebe, Meike; Wagner, Natalia; Neve, Horst; Franz, Charles M.A.P.; Hinrichs, Jörg; Atamer, Zeynep
• Is Part Of: International dairy journal. Volume 122(2021)
• Publication Date: 2021-11
• Subjects: Dairying -- Periodicals; Industrie laitière -- Périodiques; Electronic journals; 338.1762142
• DOI: 10.1016/j.idairyj.2021.105149
• Languages: English
• ISSNs: 0958-6946

Abstract

Abstract: Lactococcal bacteriophages can cause fermentation failures in the cheese production (e.g., Cheddar), especially when the by-product whey (≤10 9 plaque-forming units mL −1 of bacteriophages) is recycled and added to new batches. Using membrane filtration, the bacteriophage titre in whey can be reduced by 4 log units without protein denaturation. To achieve higher bacteriophage reduction (≥9 log units), we attempted an orthogonal process strategy (combination of different technologies supporting each other). Three representative bacteriophages were inactivated in membrane filtered whey by (i) UV-C irradiation and (ii) heat treatment to achieve additional 5 log units reduction. Thermal treatment was only partially successful, as the varying degrees of heat resistance required, in the worst-case, temperature-time combinations above pasteurisation conditions. In contrast, UV-C treatment required doses of maximally 2.25 J cm −2 for successful inactivation, thus representing a promising avenue for the establishment of a novel process for the production of "phage-free" whey.