Inhibition of off-target cleavage by RNase H using an artificial cationic oligosaccharide. Issue 31 (29th July 2021)

• Record Type: Journal Article
• Authors: Hara, Rintaro Iwata; Wada, Takeshi
• Is Part Of: Organic & biomolecular chemistry. Volume 19:Issue 31(2021)
• Page Start: 6865
• Page End: 6870
• Publication Date: 2021-07-29
• Subjects: Chemistry, Organic -- Periodicals; Bioorganic chemistry -- Periodicals; Chemistry, Physical organic -- Periodicals; 547
• DOI: 10.1039/d1ob00983d
• Languages: English
• ISSNs: 1477-0520

Abstract

Abstract : We describe a novel strategy to inhibit off-target cleavage by RNase H using an artificial cationic oligosaccharide, which bind to DNA/RNA hybrid duplexes. Abstract : Sequence-dependent off-target effects are a serious problem of antisense oligonucleotide-based drugs. Some of these side effects are induced by ribonuclease H (RNase H)-mediated cleavage of non-target RNAs with base sequences similar to that of the target RNA. We found that an artificial cationic oligosaccharide, ODAGal4, improved single-base discrimination for RNase H cleavage.