Effects of Selenium-enriched probiotics on ochratoxin A-induced kidney injury and DNMTs expressions in piglets. (October 2021)

• Record Type: Journal Article
• Authors: Gan, Fang; Hou, Lili; Lin, Ziman; Ge, Lei; Liu, Dandan; Li, Haolei; Chen, Xingxiang; Huang, Kehe
• Is Part Of: Research in veterinary science. Volume 139(2021)
• Page Start: 94
• Page End: 101
• Publication Date: 2021-10
• Subjects: Selenium-enriched probiotics -- Ochratoxin A -- Kidney injury -- Antioxidant status -- DNMTs -- Piglet; Veterinary medicine -- Periodicals; Veterinary Medicine -- Periodicals; Médecine vétérinaire -- Périodiques; Médecine vétérinaire -- Recherche -- Périodiques; Diergeneeskunde; 636.089
• DOI: 10.1016/j.rvsc.2021.07.007
• Languages: English
• ISSNs: 0034-5288

Abstract

Abstract: Effects of Selenium-enriched probiotics (SP) on ochratoxin A-induced kidney injury, growth performance, antioxidant injury, selenoprotein and DNA methylation transferases (DNMTs) expression of piglets were investigated in the article. A total of 48 piglets were randomly divided into 4 groups and fed with basal diet (Con, 0.15 mg Se/kg and OTA at 0.00 mg/kg), basal diets added with OTA (OTA, 0.40 mg OTA/kg), SP and OTA (SP1, 0.15 mg Se/kg and 0.40 mg OTA/kg), SP and OTA (SP2, 0.30 mg Se/kg and 0.40 mg OTA/kg) respectively for 42 days. From each group, six piglets were randomly selected for blood collection on Days 0 and 42 and three piglets were selected for tissue collection on Day 42.The results showed that OTA at 0.40 mg /kg significantly decreased growth performance of pigs, induced the histopathological lesions of kidney and increased urea and creatine levels of serum, decreased GPx and SOD activities, and increased MDA levels. OTA decreased GPx1, GPx4 and SelS expressions, and increased TR1, DNMT 1, DNMT3a and SOCS3 expressions. Both SP1 and SP2 improved OTA-induced poor growth performance, kidney injury, poor antioxidant statues, GPx1, SelS, TR1, SOCS3, DNMT1 and DNMT3a expressions in kidney of pigs. The effects of SP2 on the above parameters changes were better than that of SP1. SP increased GPx and SOD activities and decreased MDA levels changes induced by OTA treatment. These results suggest that SP may serve as a better feed additive for piglets under mycotoxin contamination environments. Highlights: OTA induced kidney injury and DNMTs expression of piglet. Selenium-enriched probiotics improved OTA-induced antioxidant injury and selenoproteins mRNA expressions . Selenium-enriched probiotics reversed the DNMT 1 and DNMT 3a expression induced by OTA . Selenium-enriched probiotics improved OTA-induced kidney injury .